Abstract
SCULPTURE patterns of the eggs of mosquitoes have been used for identification of closely related species by Horsfall, Miles and Skoatch1 and Craig and Horsfall2,3. The technique used by these authors was outlined by Craig4. It involved first the removal of the exochorion by abrasive action followed by the bleaching of the endochorion. The endochorionic patterns revealed by these treatments were recorded by phase contrast photography.
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References
Horsfall, W. R., Miles, R. C., and Skoatch, J. T., Ann. Entomol. Soc. Amer., 44, 618 (1952).
Craig, jun., J. B., and Horsfall, W. R., Ann. Entomol. Soc. Amer., 49, 368 (1956).
Craig, jun., J. B., and Horsfall, W. R., Ann. Entomol. Soc. Amer., 53, 11 (1960).
Craig, jun., J. B., Mosq. News, 15, 228 (1955).
MaClung's Handbook of Microscopical Technique, third ed. (Hoeber, New York, 1950).
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PILLAI, S. A Celloidin Impression Technique for Recording Egg Sculpturing in Aëdes Mosquitoes. Nature 194, 212–213 (1962). https://doi.org/10.1038/194212a0
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DOI: https://doi.org/10.1038/194212a0
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