Abstract
PRESENT methods for the production of Clostridium histolyticum collagenase depend chiefly on growth of the organism in complex culture media1,2. It is the purpose of this communication to describe a relatively simple, partially defined medium for the production of large quantities of highly active fermentation broths of Cl. histolyticum collagenase. The medium described appeared to facilitate the isolation and purification of collagenases which were essentially free of non-specific proteinases3.
Similar content being viewed by others
References
MacLennan, J. D., Mandl, I., and Howes, E. L., J. Clin. Invest., 32, 1317 (1953).
Lytell, A. A., and Hewson, K., Proc. Soc. Exp. Biol. and Med., 74, 555 (1950).
Grant, N. A., and Alburn, H., Arch. Biochem. Biophys., 82, 245 (1959).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
WARREN, G., GRAY, J. A Simplified Culture Medium for the Production of Collagenase. Nature 192, 755–756 (1961). https://doi.org/10.1038/192755a0
Issue Date:
DOI: https://doi.org/10.1038/192755a0
- Springer Nature Limited
This article is cited by
-
Glycine fermentation by Clostridium histolyticum
Archives of Microbiology (1988)