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Observations on Globin, Hæmoglobin and Haptoglobin using a Rabbit Antiglobin Serum

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Abstract

AN antiserum against human globin was raised in a rabbit by immunization with globin prepared from adult hæmoglobin by the methods of Anson and Mirsky1 and Jope, Jope and O'Brien2. A 1 per cent solution was injected subcutaneously in Freund's adjuvant3, and intravenously. The resulting antiserum precipitated both human globin and human hæmoglobin; 1 ml. precipitated about 0.5 mgm. of hæmoglobin.

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References

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ROWE, D., SOOTHILL, J. Observations on Globin, Hæmoglobin and Haptoglobin using a Rabbit Antiglobin Serum. Nature 186, 975–976 (1960). https://doi.org/10.1038/186975a0

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