Benedict's Solution as a Developer in Carbohydrate Chromatography

Abstract

WHILE a large number of reagents have been developed for the differentiation of carbohydrates by chromatography, there is still some difficulty in showing reducing sugars in many biological materials. Many of the developers, such as silver nitrate, tend to become easily reduced and to stain the whole paper, the carbohydrates being shown as dark spots on a similarly stained background. This background stain can be removed¹, but the process is both time-consuming and cumbersome.