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A Rapid Method for determining the Concentration of Ammonium Sulphate Solutions

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Abstract

THE separation of proteins and enzymes by the ammonium sulphate gradient elution technique has been reported by a number of workers1–4. Dixon5 and Kunitz6 have published a useful nomogram and equation, respectively, for use in the preparation of ammonium sulphate solutions. While the concentration of ammonium sulphate in a solution can be determined by the usual methods (that is, by distillation and titration, by specific-gravity determinations, nesslerization, or by gravimetric methods) they are all time-consuming, often involve large dilutions of the sample and, in general, are not useful for the routine analysis of a large number of samples. The use of refractometry has been investigated in the search for a more rapid method of measuring the concentration of ammonium sulphate in the fractions collected by the gradient elution technique as well as in the classical precipitation method.

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References

  1. Sayre, F. M., Hill, B. R., and Reinhardt, L. G., Proc. Soc. Exp. Biol. Med., 96, 695 (1957).

    Article  CAS  Google Scholar 

  2. Zittle, C. A., and Della Monica, E. S., Arch. Biochem. Biophys., 58, 31 (1955).

    Article  CAS  Google Scholar 

  3. Schwimmer, S., Nature, 171, 443 (1953).

    Article  ADS  Google Scholar 

  4. Zahl, R. K., and Stahl, I., Z. physiol. Chem., 293, 1 (1953).

    Article  Google Scholar 

  5. Dixon, M., Biochem. J., 54, 457 (1953).

    Article  CAS  Google Scholar 

  6. Kunitz, M., J. Gen. Physiol., 35, 423 (1952).

    Article  CAS  Google Scholar 

  7. Kirk, P. L., “Adv. Protein Chem.”, 3, 154 (1947).

    Google Scholar 

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Authors and Affiliations

  1. Department of Food Technology, University of California, Davis, California

    JOHN R. WHITAKER

  2. Department of Agricultural Chemistry, Oregon State College, Corvallis, Oregon

    ROBERT HUGHES

Authors
  1. JOHN R. WHITAKER
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  2. ROBERT HUGHES
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WHITAKER, J., HUGHES, R. A Rapid Method for determining the Concentration of Ammonium Sulphate Solutions. Nature 183, 603–604 (1959). https://doi.org/10.1038/183603a0

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