Abstract
SYNTHESIS of high-energy phosphate bonds was measured by the estimation of phosphorus: oxygen ratios. The materials used were 7–9 day old chick embryos of a hybrid White Leghorn ♀ × New Hampshire Red ♂. Except for the step isocitrate→ succinate, the estimations were done on the mitochondrial fraction, prepared according to the method of Hogeboom and Schneider1. Oxygen consumption was measured by the Warburg technique, using 0.1 M phosphate buffer at pH 7.4 with the addition of adequate co-factors and substrates2. Phosphorus estimations were carried out according to the method of Lowry and Lopez3.
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References
Hogeboom, G. H., “Methods in Enzymology”, edit. by Colowick and Kaplan, 1, 16 (Academic Press, New York, 1955).
Hunter, F. E., “Phosphorus Metabolism”, edit. by McElroy and Glass, 1, 297 (1951).
Lowry, O. H., and Lopez, J. A., J. Biol. Chem., 162, 421 (1946).
Copenhaver, J. H., and Lardy, H. A., J. Biol. Chem., 195, 225 (1952).
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NOWINSKI, W., MAHAFFEY, W. Oxidative Phosphorylation in the Chick Embryo. Nature 183, 45–46 (1959). https://doi.org/10.1038/183045a0
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DOI: https://doi.org/10.1038/183045a0
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