Abstract
A COMPLICATION often encountered in the use of the fluorescent antibody technique of Coons and Kaplan1 is the presence of non-specific staining material in the conjugates, even after exhaustive dialysis. Apart from its nuisance-value, the effect is of interest because it does not occur when fluorescein-globulin reacts with sections of various malignant tissues2,3.
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References
Coons, A. H., and Kaplan, M. H., J. Exp. Med., 91, 1 (1950).
Hughes, P. E., Louis, C. J., Dineen, J. K., and Spector, W. C., Nature, 180, 289 (1957).
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Oudin, J., “Methods in Medical Research”, 5, pp. 340–359 (Year Book Publishers, Chicago, 1952).
Raymond, S., Proc. Soc. Exp. Biol. Med. (N.Y.), 81, 278 (1952).
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CURTAIN, C. Electrophoresis of Fluorescent Antibody. Nature 182, 1305–1306 (1958). https://doi.org/10.1038/1821305a0
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DOI: https://doi.org/10.1038/1821305a0
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