Abstract
LIVER cells have been isolated by various methods, such as by forcing the tissue through stainless steel sieves1, by tryptic digestion2 and by incubation in acid solutions3. Anderson4 obtained a high yield of cells by perfusion of liver with calcium-free solutions containing chelating agents (such as ethylenediamine tetraacetate (versene) or citrate) and subsequent dispersion.
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References
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BRANSTER, M., MORTON, R. Isolation of Intact Liver Cells. Nature 180, 1283–1284 (1957). https://doi.org/10.1038/1801283a0
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DOI: https://doi.org/10.1038/1801283a0
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