Reconstruction of Coupled Phosphorylation in the Succinic Oxidase System

Abstract

CALCIUM ions are known to inhibit oxidative phosphorylation in isolated mitochondria. As reported previously¹, in the case of substrates oxidized via diphosphopyridine nucleotide, this inhibition can be reversed by the addition of the pyridine nucleotide, adenosine triphosphate and manganous ions. Treatment with the pyridine nucleotide alone partially restores only the respiration; but addition of all three factors is necessary in order to re-establish both oxidation and phosphorylation.