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Detection of Adenine Carbon-14 in Deoxyribonucleic Acid by Autoradiography

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Abstract

THE standard procedure for detecting phosphorus-32 in deoxyribonucleic acid by autoradiography is to hydrolyse the smears or sections in N HCl at 60° C. for 6–10 minutes. The acid hydrolysis removes RNA and most of the non-DNA phosphates.

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References

  1. Lajtha, L. G., J. Clin. Path., 5, 67 (1952); Exp. Cell Res., 3, 696 (1952).

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Authors and Affiliations

  1. Department of Radiotherapy, Oxford

    L. G. LAJTHA

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  1. L. G. LAJTHA
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LAJTHA, L. Detection of Adenine Carbon-14 in Deoxyribonucleic Acid by Autoradiography. Nature 173, 587–588 (1954). https://doi.org/10.1038/173587a0

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