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Artefacts produced from 3-Iodo-Tyrosine and 3 : 5-Di-iodo-Tyrosine by Heating with Alkali

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Abstract

DURING a study1 of the hydrolysis of native hog thyroglobulin labelled with iodine-131, by a purified protease isolated from hog thyroid glands, fractions eluted from paper chromatograms of the enzyme fission products were treated with 2N sodium hydroxide at 105° for 16 hr. Control aliquots were allowed to stand for the same time at 0°. On application of n-butanol extracts of the heated and control samples to paper and development of the chromatograms with n-butanol – n-pentanol – ammonia2 as solvent, unexpected radioactive spots of high RF (at 0.52 and 0.68) were observed with the heated samples. Since the original eluates were found to contain free mono- and di-iodo-tyrosine and thyroxine, the action of alkali on purified samples of the iodo-amino-acids was examined.

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References

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Authors and Affiliations

  1. Department of Biochemistry, University of Melbourne, Melbourne, N.3

    P. G. STANLEY

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  1. P. G. STANLEY
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STANLEY, P. Artefacts produced from 3-Iodo-Tyrosine and 3 : 5-Di-iodo-Tyrosine by Heating with Alkali. Nature 171, 933–934 (1953). https://doi.org/10.1038/171933b0

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