Abstract
THE proteins bovine γ-globulin and human serum albumin were found to give precipitates with the polyhaptenic dyes, 2-methyl-4,6-di(p-azobenzenearsonic acid) phenol (III), and l,3-dihydroxy-2,4,6-tri(p-azobenzenearsonic acid) benzene (VI)1, from pH 3.72 to pH. 0.2–0.3 above the isoelectric points of the proteins, and in sodium acetate – acetic acid buffer of ionic strength either 0.20 or 0.10. Either 0.5 or 1.0 ml. protein solution was added to 0.5 or 1.0 ml. of each of a series of serially diluted dye solutions of initial concentrations of 0.57 (III) and 0.80 per cent (VI), the serial dilutions being made with buffer of the same pH and ionic strength as the protein solution.
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References
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MORRISON, J. Non-specific Precipitation of Proteins by Polyhaptenic Dyes. Nature 171, 346–347 (1953). https://doi.org/10.1038/171346b0
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DOI: https://doi.org/10.1038/171346b0
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