Growth-Factor Requirements of Pellicularia koleroga Cooke in Pure Culture

Abstract

A PURE culture (isolate 1) of Pellicularia koleroga¹ (Corticium koleroga) was isolated from the basidiospores found on Coffea arabica leaves, following well-known methods². The fungus grew well on a number of agar media containing fresh extracts of potato, onion, carrot, oat, bean and malt, whereas its growth was very poor on a dextrose nitrate medium. However, when small quantities of malt, yeast, coffee leaf or fungal mat extracts were added to the dextrose nitrate medium, the fungus made good growth. A culture of this fungus obtained from the Centraal-bureau voor Schimmelcultures, Baarn, Holland, also behaved similarly, and its growth was even slower. It was therefore felt that Pellicularia koleroga may have certain growth-factor requirements.