Abstract
THE isolation of more abundant natural compounds mostly uses very simple means—extraction, distiltion, crystallization—and combinations and refinements of these methods. The problem of isolatiing, less abundant natural substances is associated with two outstanding difficulties. The scale of the extraction process is much greater, and the desired material is often accompanied by a variety of very similar materials. Without doubt chromatography has done more than any other experimental advance to overcome these difficulties.
Similar content being viewed by others
References
Tswett, M., Ber. deutsch. Bot. Ges., 24, 316 (1906).
Symposium, Ann. N.Y. Acad. Sci., 49, 141 (1948).
Brockmann, H., et al., Naturwiss, 33, 58 (1946); Ber., 80, 77 (1947); Angew. Chem., 59, 30 (1947).
Sease, J. W., J. Amer. Chem. Soc., 69, 2242 (1947).
Zeichmeister, L., Ann. N.Y. Acad. Sci., 49, 220 (1948).
See for general accounts: Tiselius, A., “Advances in Protein Chemistry", 3, 67 (1947). Claesson, S., Ann. N.Y. Acad. Sci., 49, 183 (1948).
Cannan, R. K., Ann. N.Y. Acad. Sci., 47, 135 (1946). Appelweig, ibid., 49, 295 (1948).
Cohn, W. E., Science, 109, 377 (1949).
Martin, A. J. P., and Synge, R. L. M., Biochem. J., 35, 1358 (1941).
Moyle, V., Baldwin, E., and Scarisbrick, R., Biochem. J., 43, 308 (1948).
"The Chemistry of Penicillin", 76, 38.
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).
Williams, F. J., and Kirby, H., Science, 107, 481 (1948).
Consden, R., Nature, 162, 359 (1948).
Sanger, F., Biochem. J., 42, 287 (1948). Paterson et al., J. Biol. Chem., 74, 775 (1948).
Dent, C. E., Biochem. J., 43, 169 (1948).
See, for example, Hamer, D., and Woodhouse, D. L., Nature, 163, 689 (1949).
Dent, C. E., Biochem. J., 41, 240 (1947).
Polson, A., Mosley, V. M., and Wyckoff, R. W. C., Science, 105, 603 (1947). Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem, J., 42, 443 (1948).
Woiwod, A. J., Nature, 161, 169 (1948).
Keston, A. S., Udenfriend, S., and Levy, M., J. Amer. Chem. Soc., 69, 3151 (1947).
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 41, 590 (1947).
Consden, R., Gordon, A. H., Martin, A. J. P., and Synge, R. L. M., Biochem. J., 41, 596 (1947).
Bate-Smith, E. C., Nature, 161, 835 (1948).
Goodall, R. R., and Levi, A. A., Nature, 158, 674 (1946).
Vischer, E., and Chargaff, E., J. Biol. Chem., 168, 781 (1947).
Partridge, S. M., Biochem. J., 42, 238, 251 (1948). Forsyth, W. G. C. Nature, 161, 239 (1948).
Chargaff, E., et al., J. Biol. Chem., 175, 67 (1948).
Haller, H. C., Acree, F., and Potts, S. F., J. Amer. Chem. Soc., 66, 1659 (1944).
Bentley, F. R., McDermott, E. E., Pace, J., Whitehead, J. K., and Moran, T., Nature, 163, 765 (1949).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
COOK, A. Chromatographic Separation of Natural Compounds. Nature 164, 300–302 (1949). https://doi.org/10.1038/164300a0
Issue Date:
DOI: https://doi.org/10.1038/164300a0
- Springer Nature Limited