Golgi Apparatus of Living Human Testicular Cells Seen with Phase-Contrast Microscopy

Abstract

IT was pointed out previously that in the unstained living human spermatid the Golgi apparatus is sometimes discernible with bright-ground illumination, as an area slightly differing in refractive index from the remainder of the cytoplasm¹. Recently, I have been able to confirm and extend these observations with the phase-contrast microscope. This instrument was of the phase-accelerating or positive type, so that dark and light regions in the image indicated higher and lower refractive indices, as a rule.