Abstract
OF various media used in artificial insemination or the preservation of the semen1, the egg-yolk phosphate mixture of Phillips and Lardy2 has given the best results, maintaining the fertilizing capacity of bull semen for several days at 5° C. Egg-yolk phosphate is also a good medium for maintaining oxygen uptake of the semen in respiratory experiments, and Walton and Edwards3 found a positive correlation between oxygen uptake of semen samples and the fertility of the bulls from which the samples were taken. Experiments have therefore been undertaken to determine the factors present in the egg-yolk affecting the respiratory activity at 37° C., in expectation that the knowledge gained will assist in the discovery of the most favourable conditions of storage.
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References
For a review see Anderson, J., "The Semen of Animals and its Use in Artificial Insemination" (Edinburgh: Imperial Bureau of Animal Genetics, 1945).
Phillips, P. H., and Lardy, H. A., J. Dairy Sci., 23, 399 (1940).
Walton, A., and Edwards, J., Proc. Amer. Soc. Anim. Prod., 31st Ann. Meet., 254 (1938).
Walton, A., "The Technique of Artificial Insemination" (London: Holborn Surgical Instrument Co., 1945), in the press.
Dixon, M., "Manometric Methods" (2nd edit., Cambridge, 1944), 6.
Keilin, D., and Hartree, E. F., Proc. Roy. Soc., B, 119, 141 (1936).
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TOŠIÓ, J., WALTON, A. Respiration of Spermatozoa in Egg-Yolk Medium. Nature 156, 507–508 (1945). https://doi.org/10.1038/156507b0
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DOI: https://doi.org/10.1038/156507b0
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