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X-Ray Induced Mutations in the Physiology of Ophiostoma

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Abstract

Tatum's and Beadle's1 investigations on Neurospora inspired me to attempt to induce physiological mutations in Ophiostoma multiannulatum (Hedge, and Davids.) by means of X-ray treatment. Normal strains of this fungus grew optimally on a synthetic medium containing glucose, ammonium tartrate, inorganic salts, aneurin (= vitamin B1) and pyridoxin (= vitamin B6)2. Out of 775 monosporous mycelia which were obtained from X-ray treated ascospores by means of a special procedure3, 12 (that is, 1·6 per cent) differed physiologically. The manner in which they differed is shown in the accompanying table.

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References

  1. Beadle G. W. and Tatum E. L., Nat. Acad. Sci. Proc., 27, 499 (1941). Tatum E. L. and Beadle G. W., ibid., 28, 234 (1942). (only available in abstracts.)

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  2. Fries N., Symb. Bot. Upsal., 7, 2 (1943).

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  3. Fries N., Ark. Bot., 32, 4 (1945).

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  4. Volkonsky M., C.R. Soc. Biol. Paris, 109, 528 (1932).

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  5. Fildes P. and Richardson G. M., Brit. J. Exp. Pathol., 18, 292 (1937).

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FRIES, N. X-Ray Induced Mutations in the Physiology of Ophiostoma. Nature 155, 757–758 (1945). https://doi.org/10.1038/155757a0

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