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A high-density probe array sample preparation method using 10- to 100-fold fewer cells

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Figure 1: Sample preparation method begins with total RNA extraction from cells or tissues.
Figure 2: (A) Number and expression level of transcripts detected in duplicate samples prepared by poly(A) and total RNA methods.
Figure 3: Images of hybridizations of 2.5, 5.0, 10.0, and 20.0 μg of labeled target from total RNA.

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Acknowledgements

We thank D. Lockhart, S. Dee, and N. Shah for helpful discussions during the course of this work. We thank S.Carter and A. Lau for aid in manuscript preparation. GeneChip is a registered trademark of Affymetrix, Inc.

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Authors and Affiliations

  1. research associate, Affymetrix, Inc., 3380 Central Expressway, Santa Clara, 95051, CA

    Mamatha Mahadevappa

  2. director of high-throughput screening, Affymetrix, Inc., 3380 Central Expressway, Santa Clara, 95051, CA

    Janet A. Warrington

Authors
  1. Mamatha Mahadevappa
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  2. Janet A. Warrington
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Mahadevappa, M., Warrington, J. A high-density probe array sample preparation method using 10- to 100-fold fewer cells. Nat Biotechnol 17, 1134–1136 (1999). https://doi.org/10.1038/15124

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