Advertisement

Exopectinases produced by Aspergillus niger in solid-state and submerged fermentation: a comparative study

  • G Díaz-Godínez
  • J Soriano-Santos
  • C Augur
  • G Viniegra-González

Exopectinase production by Aspergillus niger was compared in submerged fermentation (SmF) and solid-state fermentation (SSF). SSF was carried out using polyurethane foam (PUF) as the solid support. The purpose was to study the effect of sucrose addition (0 or 40 g/l) and water activity level (Aw=0.99 or 0.96) on the level of enzyme activity induced by 15 g/l of pectin. Mycelial growth, as well as extracellular protease production, was also monitored. Sucrose addition in SmF resulted in catabolite repression of exopectinase activity. However, in SSF, an enhancement of enzyme activity was observed. Protease levels were minimal in SSF experiments with sucrose and maximal in SmF without sucrose. Exopectinase yields (IU/g X) were negligible in SmF with sucrose. The high levels of exopectinase with sucrose and high Aw in SSF can be explained by a much higher level of biomass production without catabolite repression and with lower protease contamination. Journal of Industrial Microbiology & Biotechnology (2001) 26, 271–275.

Keywords: exopectinase; solid-state fermentation; polyurethane; enzyme yield 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Society for Industrial Microbiology 2001

Authors and Affiliations

  • G Díaz-Godínez
    • 1
  • J Soriano-Santos
    • 2
  • C Augur
    • 3
  • G Viniegra-González
    • 2
  1. 1.Área de Biotecnología del Centro de Investigación en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Ixtacuixtla Tlaxala, Me´xicoMX
  2. 2.Departamento de Biotecnología, Universidad Autónoma Metropolitana, Av. Michoacán y La Purísima, Me´xico, DF, CP 09340, Me´xicoMX
  3. 3.IRD-Mexique, Cicerón 609, Los Morales 11530, Me´xico, DF, Me´xicoMX

Personalised recommendations