Residual phosphate concentration under nitrogen-limiting conditions regulates curdlan production in Agrobacterium species

  • M-K Kim
  • I-Y Lee
  • J-H Lee
  • K-T Kim
  • Y-H Rhee
  • Y-H Park

We investigated the influence of inorganic phosphate concentration on the production of curdlan by Agrobacterium species. A two-step culture method was employed where cells were first cultured, followed by curdlan production under nitrogen-limiting conditions. In the curdlan production step, cells did not grow but metabolized sugar into curdlan. Shake-flask experiments showed that the optimal phosphate concentration for curdlan production was in the range of 0.1–0.3 g l−1. As the cell concentration increased from 0.42 to 1.68 g l−1 in shake-flask cultures, curdlan production increased from 0.44 to 2.80 g l−1. However, the optimal phosphate concentration range was not dependent upon cell concentration. The specific production rate was about 70 mg curdlan g-cell−1 h−1 irrespective of cell concentration. When the phosphate concentration was maintained at 0.5 g l−1 under nitrogen-limiting conditions, as high as 65 g l−1 of curdlan was obtained in 120 h. Journal of Industrial Microbiology & Biotechnology (2000) 25, 180–183.

Keywords: curdlan; production; phosphate concentration; Agrobacterium sp 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Society for Industrial Microbiology 2000

Authors and Affiliations

  • M-K Kim
    • 1
  • I-Y Lee
    • 1
  • J-H Lee
    • 2
  • K-T Kim
    • 1
  • Y-H Rhee
    • 3
  • Y-H Park
    • 1
  1. 1.Microbial and Bioprocess Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yusong, Taejon 305-600, South KoreaKR
  2. 2.Department of Chemical Engineering, Chosun University, 501 Kwangju, South Korea
  3. 3.Department of Microbiology, Chungnam National University, 220 Kungdong, Taejon 305-764, South Korea

Personalised recommendations