Journal of Industrial Microbiology and Biotechnology

, Volume 23, Issue 3, pp 155–165

Protein diffusion in alginate beads monitored by confocal microscopy. The application of wavelets for data reconstruction and analysis

  • A Laca
  • L A García
  • F Argüeso
  • M Díaz

A microscopic technique has been developed to obtain the protein profiles inside calcium alginate gel. To do this, the diffusion of BSA, previously marked with FITC, inside calcium alginate beads was observed using confocal laser microscopy, thus obtaining the spatio-temporal evolution of the protein concentration. The technique, however, presents certain limitations and zones where it is impossible to obtain experimental data. Wavelets analysis, commonly used in signal processing and statistics, was employed to reconstruct and subsequently analyse the experimental results. Once the diffusion model was defined, the substrate profiles obtained were used to calculate a diffusivity value for BSA in alginate gel.

Keywords: diffusion; BSA; alginate; wavelets 

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Copyright information

© Society for Industrial Microbiology 1999

Authors and Affiliations

  • A Laca
    • 1
  • L A García
    • 1
  • F Argüeso
    • 2
  • M Díaz
    • 1
  1. 1.Dept of Chemical Engineering and Environmental Technology (IUBA), University of Oviedo, c/Julián Clavería s/n, 33071 Oviedo, SpainES
  2. 2.Dept of Mathematics, Faculty of Sciences, University of Oviedo, c/Calvo Sotelo s/n, 33007 Oviedo, SpainES

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