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Lipase from Pseudomonas aeruginosa LP602: biochemical properties and application for wastewater treatment

  • S Dharmsthiti
  • B Kuhasuntisuk

Lipase from Pseudomonas aeruginosa LP602, a bacterial strain isolated from a domestic wastewater sample, was preliminarily characterized. The enzyme exhibited maximum lipolytic activity at pH 8.0 where it was also stably maintained. At 55°C, the lipase had the highest activity but not stability. The enzyme was insensitive to EDTA and to many ions tested except Zn2+. It was sensitive to SDS but not to Tween-20, Tween-80 or Triton X-100. The enzyme was active towards a number of commercial food grade fats and oils. A suitable medium formula for lipase production was MMP containing 6.25% whey as a carbon source, 1% soybean oil as inducer and 0.5% yeast extract supplement. The culture was fed with glucose to a final concentration of 0.1% at the 15th hour of incubation. Lipase production under this condition was 3.5 U ml−1. Both P. aeruginosa LP602 cells and the lipase were shown to be usable for lipid-rich wastewater treatment.

Keywords: lipase; Pseudomonas aeruginosa; wastewater treatment 

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Copyright information

© Society for Industrial Microbiology 1997

Authors and Affiliations

  • S Dharmsthiti
    • 1
  • B Kuhasuntisuk
    • 1
  1. 1.Center for Biotechnology, Institute for Research and Development in Science and Technology, Mahidol University, Bhuddhamonthon 4 Rd, Salaya, Nakornpathom 73170, ThailandTH

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