Reduction of Cr, Mo, Se and U by Desulfovibrio desulfuricans immobilized in polyacrylamide gels

  • M D Tucker
  • L L Barton
  • B M Thomson

Intact cells of Desulfovibrio desulfuricans, immobilized in polyacrylamide gel, removed Cr, Mo, Se and U from solution by enzymatic-mediated reduction reactions. Lactate or H2 served as the electron donor and the oxidized Cr(VI), Mo(VI), Se(VI) and U(VI) served as electron acceptors. Reduction of the oxidized metal species resulted in the precipitation of solid phases of the metals. Metal removal efficiencies of 86–96% were achieved for initial concentrations of 1 mM Mo, Se, and U and 0.5 mM Cr. Insoluble metal phases accumulated on both the surface and the interior of the polyacrylamide gel. In column tests conducted for U removal, effluent concentrations less than 20 μg L−1 were achieved with initial concentrations of 5 mg L−1 and 20 mg L−1 U and residence times from 25–37 h. The enzymatic reduction of Cr, Mo, Se, and U by immobilized cells of D. desulfuricans may be a practical method for removing these metals from solution in a biological reactor.

Keywords: immobilized cells; Desulfovibrio; dissimilatory metal reduction 

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Copyright information

© Society for Industrial Microbiology 1998

Authors and Affiliations

  • M D Tucker
    • 1
  • L L Barton
    • 2
  • B M Thomson
    • 3
  1. 1.Sandia National Laboratories, Albuquerque, NM 87185, USAUS
  2. 2.Department of Biology, University of New Mexico, Albuquerque, NM 87131, USAUS
  3. 3.Department of Civil Engineering, University of New Mexico, Albuquerque, NM 87131, USAUS

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