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Role of Nitric Oxide in Oxidative Damage in Isolated Rabbit Gastric Cells Exposed to Hypoxia-Reoxygenation

Abstract

The present study aimed to investigate the roleof nitric oxide on the oxidative damage in isolatedrabbit gastric cells exposed to hypoxia-reoxygenation.Nitric oxide synthesis modulators such as L-arginine and NG-nitro-L-arginine methylester, a nitric oxide donor, sodium nitroprusside, andsuperoxide dismutase were used to treat the cells, andthe synthesis and secretion of mucus, lipid peroxideproduction, and glutathione contents of the cells weredetermined. As a result, hypoxia-reoxygenation decreasednitric oxide production and the synthesis and secretionof mucus, as well as glutathione contents of gastric cells, but hypoxia-reoxygenation increasedlipid peroxide production. Pretreatment with L-arginine,a substrate for nitric oxide synthase, sodiumnitroprusside, and superoxide dismutase prevented theincrease in lipid peroxide production and the decreasesin glutathione contents, as well as the synthesis andsecretion of mucus induced by hypoxia-reoxygenation.However, NG-nitro-L-arginine methyl ester, anitric oxide synthase inhibitor, had no effect onthese alterations. In conclusion, nitric oxide has anantioxidant defensive role on gastric cells bymaintaining mucus and glutathione.

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Kim, H., Kim, K.H. Role of Nitric Oxide in Oxidative Damage in Isolated Rabbit Gastric Cells Exposed to Hypoxia-Reoxygenation. Dig Dis Sci 43, 1042–1049 (1998). https://doi.org/10.1023/A:1018886919532

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  • DOI: https://doi.org/10.1023/A:1018886919532

  • NITRIC OXIDE
  • MUCUS
  • LIPID PEROXIDE
  • GLUTATHIONE
  • GASTRIC CELL
  • HYPOXIA/REOXYGENATION