Abstract
Flow treatment of the yeast, Schizosaccharomyces pombe, with high intensity electric field pulses released intracellular enzymes such as glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase. Over 70% of the total activity was liberated within 4 h after pulse application. The optimal field intensities were considerably higher than that needed for irreversible plasma membrane permeabilization.
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Ganeva, V., Galutzov, B. & Teissié, J. Flow process for electroextraction of intracellular enzymes from the fission yeast, Schizosaccharomyces pombe . Biotechnology Letters 26, 933–937 (2004). https://doi.org/10.1023/B:bile.0000025906.95694.1a
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DOI: https://doi.org/10.1023/B:bile.0000025906.95694.1a