Ngari, hentak and tungtap are traditional fermented fish products of North-East India. Eighteen samples of ngari, hentak and tungtap were collected and were analysed for microbial load. Lactic acid bacteria, endospore-forming rods, yeasts and aerobic mesophilic counts ranged from 4.0 to 7.2, 3.3–4.6, <1–3.5 and 4.3–7.3 log c.f.u./g, respectively. Lactic acid bacteria were identified as Lactococcus lactis subsp. cremoris, Lactococcus plantarum, Enterococcus faecium, Lactobacillus fructosus, Lactobacillus amylophilus, Lactobacillus coryniformis subsp. torquens and Lactobacillus plantarum. Endospore-forming rods were identified as Bacillus subtilis and Bacillus pumilus, aerobic coccal strains were identified as Micrococcus. Yeasts were identified as species of Candida and Saccharomycopsis. Pathogenic contaminants were detected in all samples, however, none of the sample contained more than 102 c.f.u./g of Bacillus cereus, 103 c.f.u./g of Staphylococcus aureus and enterobacteriaceae population, respectively. Enzymatic and antimicrobial activities of the isolates were tested. None of the strains produced biogenic amines in the method applied. Most strains of LAB had a high degree of hydrophobicity, indicating their ‘probiotic’ characters. This study has demonstrated the microbial diversity within the species of lactic acid bacteria, Bacillus and yeasts.
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Adams, M.R. & Nicolaides, L. 1997 Review of the sensitivity of different foodborne pathogens to fermentation. Food Control 8, 227–239.
Backhoff, P.H. 1976 Some chemical changes in fish silage. Journal of Food Technology 11, 353–363.
Bartholomew, J.W. 1962 Variables influencing results and the precise definition of the steps in Gram staining as a means of standardizing the results obtained. Stain Technology 37, 139–155.
Beumer, R.R. 2001 Microbiological hazards and their control: bacteria. In Fermentation and Food Safety, eds. Adams, M.R. & Nout, M.J.R. pp. 141–157. Gaithersburg, Md., USA: Aspen Publishers, Inc. ISBN 0-8342-1843-7.
Bover-Cid, S. & Holzapfel, W.H. 1999. Improved screening procedure for biogenic amine production by lactic acid bacteria. International Journal of Food Microbiology 53, 33–41.
Claus, D. & Berkeley, R.C.W. 1986 Genus Bacillus Cohn 1872, 174. In Bergey's Manual of Systematic Bacteriology, vol. 2, eds. Sneath, P.H.A., Mair, N.S., Sharpe, M.E. & Holt, J.G. pp. 1105–1139. Baltimore, MD: Williams & Wilkins}. ISBN 0-683-07893-
Cooke, R.D., Twiddy, D.R. & Alan Reilly, P.J. 1993 Lactic fermentation of fish as a low-cost means of food preservation. In Fish Fermentation Technology, eds. Lee, C.H., Steinkraus, K.H. & Alan Reilly, P.J. pp. 291–300. Tokyo: United Nations University Press. ISBN 89-7053-93480-8.
Crisan, E.V. & Sands, A. 1975 Microflora of four fermented fish sauces. Applied Microbiology 29, 106–108.
Ding, H. & Lämmler, C. 1992 Cell surface hydrophobicity of Actinomyces pyogenes determined by hexadecane adherence-and salt aggregation studies. Zentralblatt für Veterinärmedizin (B) 39, 132–138.
Dykes, G.A., Britz, T.J. & von Holy A 1994 Numerical taxonomy and identification of lactic acid bacteria from spoiled, vacuum packaged Vienna sausages. Journal of Applied Bacteriology 76, 246–252.
Einarsson, H. & Lauzon, H.L. 1995 Biopreservation of brined shrimp (Pandalus borealis) by bacteriocins from lactic acid bacteria. Applied and Environmental Microbiology 61, 669–676.
Fuwa, H. 1954 A new method for microdetermination of amylase activity by the use of amylose as the substrate. Journal of Biochemistry 41, 583–603.
Gordon, R.E., Haynes, W.C. & Pang, C.H.-N 1973 The genus Bacillus, Handbook No. 427. Washington, DC: United States Department of Agriculture.
Han, B.Z., Beumer, R.R., Rombouts, F.M. & Nout, M.J.R. 2001 Microbiological safety and quality of commercial sufu-a Chinese fermented soybean food. Food Control 12, 541–547.
Harrigan, W.F. 1998 Laboratory Methods in Food Microbiology, 3rd edn. London: Academic Press. ISBN 0-12-326043-4.
Holzapfel, W.H., Haberer, P., Snel, J., Schillinger, U. & Huis in't Veld, J.H.J. 1998 Overview of gut flora and probiotics. International Journal of Food Microbiology 41, 85–101.
Joosten, H.M.L.J. & Northolt, M.D. 1989 Detection, growth and amine-producing capacity of lactobacilli in cheese. Applied Environmental Microbiology 55, 2356–2359.
Kandler, O. 1983 Carbohydrate metabolism in lactic acid bacteria. Antonie van Leeuwenhoek 49, 209–224.
Kawaguchi, T., Nagae, H., Murao, S. & Arai, M. 1992 Purification and some properties of a haim-sensitive α-amylase from newly isolated Bacillus sp. No 195. Bioscience, Biotechnology and Biochemistry 56, 1792–1796.
Kurtzman, C.P. & Fell, J.W. 1998 The Yeast, A Taxonomic Study, 4th edn. Amsterdam: Elsevier.
Leisner, J.J., Rusul, G., Wee, B.W., Boo, H.C. & Mohammad, K. 1997. Microbiology of Chili Bo, a popular Malaysian food ingredient. Journal of Food Protection 60, 1235–1240.
Leuschner, R.G., Kenneally, P.M. & Arendt, E.K. 1997 Method for the rapid quantitative detection of lipolytic activity among food fermenting microorganisms. International Journal of Food Microbiology 37, 237–240.
Maeda, T., Takagi, M. & Imanaka, T. 1993 Purification and characterization of a new metal protease which hydrolyzes the cyclic decapeptide, gramicidin S. Journal of Fermentation and Bioengineering 75, 173–177.
Nagai, T., Nishimura, K., Suzuki, H., Banba, Y., Sasaki, H. & Kiuchi, K. 1994 Isolation and characterization of Bacillus subtilis strain producing natto with strong Umami-taste and high viscosity. Nippon Shokuhin Kogyo Gakkaishi 41, 123–128.
Nychas, G.J.E. & Arkoudelos, J.S. 1990 Staphylococci: their role in fermented sausages. Journal of Applied Bacteriology Symposium Supplement 19, 167S–188S.
Phithakpol, B. 1993 Fish fermentation technology in Thailand. In Fish Fermentation Technology, eds. Lee, C.H., Steinkraus, K.H. & Alan Reilly, P.J. pp. 155–166. Tokyo: United Nations University Press. ISBN 89-7053-93480-8.
Rosenberg, M. 1984 Bacterial adherence to hydrocarbons: a useful technique for studying cell surface hydrophobicity. FEMS Microbiology Letters 22, 289–295.
Sarojnalini, C. & Singh, W.V. 1988 Composition and digestibility of fermented fish foods of Manipur. Journal of Food Science and Technology 25, 349–351.
Salminen, S., Isolauri, E. & Salminen, E. 1996 Clinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains for future challenges. Antonie van Leeuwenhoek 70, 347–358.
Schillinger, U. & Lücke, F.K. 1987 Identification of lactobacilli from meat and meat products. Food Microbiology 4, 199–208.
Schillinger, U. & Lücke, F.K. 1989 Antibacterial activity of Lactobacillus sake isolated from meat. Applied and Environmental Microbiology 55, 1901–1906.
Shah, N.P. 2001 Functional foods from probiotics and prebiotics. Food Technology 55, 46–53.
Slepecky, R.A. & Hemphill, H.E. 1992 The genus Bacillus-nonmedical. In The Prokaryotes, A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Application, 2nd edn, vol. 2., eds. Balows, A., Trüper, H.G., Dworkin, M., Harder, W. & Schleifer, K.H. pp. 1663–1696. New York: Springer-Verlag. ISBN 0-38797258-7.
Sneath, P.H.A., Mair, N.S., Sharpe, M.E. & Holt, J.G. 1986 Bergey's Manual of Systematic Bacteriology, vol. 2. Baltimore, MD: Williams & Wilkins. ISBN 0-683-07893-3.
Tamang, J.P. 2001 Food culture in the Eastern Himalayas. Journal of Himalayan Research and Cultural Foundation 5, 107–118.
Tamang, J.P. & Nikkuni, S. 1996 Selection of starter culture for production of kinema, a fermented soybean food of the Himalaya. World Journal of Microbiology and Biotechnology 12, 629–635.
Tanasupawat, S., Okada, S., Suzuki, K., Kozaki, M. & Komagata, K. 1992 Identification of Enterococcus hirae, E. faecalis, E. faecium, and E. casseliflavus strains from fermented foods. Bulletin of the Japan Federation for Culture Collections 8, 86–94.
Ten Brink, B., Damink, C., Joosten, H.J. & Huis in't Veld, J. 1990 Occurrence and formation of biologically active amines in foods. International Journal of Food Microbiology 11, 73–84.
Thapa, N. 2002 Studies on microbial diversity associated with some fish products of the Eastern Himalayas. PhD thesis, North Bengal University, India.
Tichaczek, P.S., Nissen-Meyer, J., Nes, I.F., Vogel, R.F. & Hammes, W.P. 1992 Characterization of the bacteriocin curvacin A from Lactobacillus curvatus LTH174 and sakacin P from Lactobacillus sake LTH673. Systematic and Applied Microbiology 15, 460–468.
Uhlman, L., Schillinger, U., Rupnow, J.R. & Holzapfel, W.H. 1992 Identification and characterization of two bacteriocin-producing strains of Lactococcus lactis isolated from vegetables. International Journal of Food Microbiology 16, 141–151.
Watanaputi, S.P., Chanyavongse, R., Tubplean, S., Tanasuphavatana, S. & Srimahasongkhraam, S. 1983 Microbiological analysis of Thai fermented foods. Journal of the Graduate School, Chulalongkorn University 4, 11–24.
Wood, B.J.B. & Holzapfel, W.H. 1995 The Lactic Acid Bacteria. vol. 2: The Genera of Lactic Acid Bacteria. London: Blackie Academic & Professional, ISBN 07514-0215-0.
Wu, Y.C., Kimura, B. & Fujii, T. 2000 Comparison of three culture methods for the identification of Micrococcus and Staphylococcus in fermented squid shiokara. Fisheries Science 66, 142–146.
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Thapa, N., Pal, J. & Tamang, J.P. Microbial Diversity in Ngari, Hentak and Tungtap, Fermented Fish Products of North-East India. World Journal of Microbiology and Biotechnology 20, 599–607 (2004). https://doi.org/10.1023/B:WIBI.0000043171.91027.7e