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Decolourization and Detoxification of Methyl Red by Aerobic Bacteria from A Wastewater Treatment Plant

World Journal of Microbiology and Biotechnology volume 20, pages 545–550 (2004)Cite this article

Abstract

Bacterial cultures from a wastewater treatment plant degraded a toxic azo dye (methyl red) by decolourization. Complete decolourization using a mixed-culture was achieved at pH 6, 30 °C within 6 h at 5 mg/l methyl red concentration, and 16 h at 20—30 mg/l. Four bacterial species were isolated that were capable of growth on methyl red as the sole carbon source, and two were identified, namely Vibrio logei and Pseudomonas nitroreducens. The Vibrio species showed the highest methyl red degradation activity at the optimum conditions of pH 6--7, and 30—35 °C. Analysis by NMR showed that previously reported degradation products 2-aminobenzoic acid and N,N-dimethyl-1,4-phenylenediamine were not observed. The decolourized dye was not toxic to a monkey kidney cell line (COS-7) at a concentration of 250 μM.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Waterloo, Ontario, Canada, N2L 3G1

    O. Adedayo, W.A. Anderson & M. Moo-Young

  2. Hormozgan University of Medical Science, Iran

    S. Javadpour

  3. Department of Biology, University of Waterloo, Ontario, Canada, N2L 3G1

    C. Taylor

Authors
  1. O. Adedayo
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  2. S. Javadpour
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  3. C. Taylor
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  4. W.A. Anderson
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  5. M. Moo-Young
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Adedayo, O., Javadpour, S., Taylor, C. et al. Decolourization and Detoxification of Methyl Red by Aerobic Bacteria from A Wastewater Treatment Plant. World Journal of Microbiology and Biotechnology 20, 545–550 (2004). https://doi.org/10.1023/B:WIBI.0000043150.37318.5f

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