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Production and Starch Saccharification by a Thermostable and Neutral Glucoamylase of a Thermophilic Mould Thermomucor Indicae-Seudaticae

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Abstract

The present investigation was aimed at producing a thermostable and neutral glucoamylase (amyloglucosidase, EC 3.2.1.3) by a thermophilic mould, Thermomucor indicae-seudaticae in submerged cultivation and testing its applicability in starch saccharification. Parametric optimization resulted in the secretion of 30,000 U/l of glucoamylase in a synthetic medium (5% soluble starch, 0.1% yeast extract, 0.05% K2HPO4 and 0.01% MgSO4· 7H2O) using 5 × 106 spores/50 ml of a 3-day-old inoculum at 40 °C and 250 rev/min in shake flasks in 48 h. The enzyme secretion was not affected to any significant extent by the tested additives and detergents. A 1.7-fold increase in glucoamylase secretion was attained when T. indicae-seudaticae was grown in a laboratory fermenter. The enzyme alone catalysed the hydrolysis of soluble starch to an extent of 65%. A prior treatment of starch with thermostable α-amylase and amylopullulanase, followed by glucoamylase, resulted in a greater extent of hydrolysis, 79 and 91%, respectively.

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  1. Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110 021, India

    Parvinder Kaur & T. Satyanarayana

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  1. Parvinder Kaur
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  2. T. Satyanarayana
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Correspondence to T. Satyanarayana.

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Kaur, P., Satyanarayana, T. Production and Starch Saccharification by a Thermostable and Neutral Glucoamylase of a Thermophilic Mould Thermomucor Indicae-Seudaticae . World Journal of Microbiology and Biotechnology 20, 419–425 (2004). https://doi.org/10.1023/B:WIBI.0000033065.22647.5b

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  • DOI: https://doi.org/10.1023/B:WIBI.0000033065.22647.5b

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