Abstract
High molecular weight lectins (> 100 kDa) from seeds of the legumes Canavalia brasiliensis (CnBr), Cratylia floribunda (CFL), Phaseolus vulgaris (PHA) and Vatairea macrocarpa (VML), temporarily stimulate the respiration of Rhizobium tropici-CIAT899 and R. etli-CFN42. These stimulants were significant (P < 0.05) in bacterial suspensions (> 2.85 mg dry biomass ml−1), having at least 6200 molecules of lectins per bacteria. The VML (20 μg ml−1), induced specific O2 demand of 2.3–2.5 ηM O2 min−1 mg dry biomass−1, in CFN42 and CIAT899, respectively. However, CnBr, CFL and PHA induced smaller demands of O2 (5×), in both strains. The order of affinities of the lectins was approximately VML > PHA > CFL > CnBr, with regard to respiratory stimuli in CIAT899 strain. The co-administration of 10 μg VML ml−1 and 9.8 ηM galactose, in CIAT899 suspensions, reduced the respiratory stimuli significantly in relation to the treatment with VML alone. These respiratory stimuli, induced by the lectins, increase the significance of the interaction lectin × Rhizobium in terms of bacterial physiology. Its understanding could be important in relation to bacterial symbiotic behaviour.
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References
Antonyuk, L.P., Fomina, O.R. & Ignatov, V.V. 1997 Effect of wheat lectin on the metabolism of Azospirillum brasiliense: induction of protein synthesis. Microbiology (Engl. transl. of Mikrobiologiya) 66, 140–145.
Booij, P., Demel, R.A., dePater, B.S. & Kijne, J.W. 1996 Insertion of pea lectin into a phospholipid monolayer. Plant Molecular Biology 31, 169–173.
Brelles-Mariño, G., Costa, G.A. & Boiardi, J.L. 1996 Enhancement of infection thread formation by Rhizobium etli incubated with bean seed lectin. Microbiological Research 151, 243–246.
Brewin, N.J. & Kardansky, I.V. 1997 Legume lectins and nodulation by Rhizobium. Trends in Plant Science 2, 92–98.
Cohn, J., Day, R.B. & Stacey, G. 1998 Legume nodule organogenesis. Trends in Plant Science 3, 105–110.
Downie, J.A. & Walker, S.A. 1999 Plant responses to nodulation factors. Current Opinion in Plant Biology 2, 483–489.
Hirsch, A.M. 1999 Role of lectins (and rhizobial exopolysaccharides) in legume nodulation. Current Opinion in Plant Biology 2, 320–326.
Kijne, J.W., Bauchrowitz, M.A. & Diaz, C.L. 1997 Root lectins and rhizobia. Plant Physiology 115, 869–873.
Lau, T.M. & Chan, K.-y. 1984 Phyiological responses of Bacillus species to Cocanavalin A: 2 effect on growth, oxygen uptake, enzyme activities and intracellular cyclic guanosine 3′, 5′-monophosphate level of B. cereus ATCC 14579. Microbios 39, 137–150.
Learemans, T. & Vanderleyden, J. 1998 Review: infection and nodulation signalling in Rhizobium-Phaseolus vulgaris symbiosis. World Journal of Microbiology and Biotechnology 14, 787–808.
Lodeiro, A.R., López-Gracía, S.L., Vázquez, T.E.E. & Favelukes, G. 2000 Stimulation of adhesiveness, infectivity, and competitiveness for nodulation of Bradyrhizobium japonicum by its pretreatment with soybean seed lectin. FEMS Microbiology Letters 188, 177–184.
Loris, R., Hamelryck, T., Bouckaert, J. & Wyns, L. 1998 Legume lectin structure. Biochimicaet Biophysica Acta 1383, 9–36.
Majumder, M., Chattopadhyay, T., Guha, A.K. & Chatterjee, B.P. 1997 Inhibition of bacterial respiration by a low-molecular weight lectin, Scyllin, from Scylla serrata crab hemolymph. Indian Journal of Biochemistry and Biophysics 34, 87–89.
Martínez, C.R. 2002 Interação Rhizobium e Lectinas de Leguminosas: Cinética de Sedimentação e Estímulos no Metabolismo Bioenergético. Doctoral Thesis, UFPE, Recife-Pernambuco, Brazil.
Mestrallet, M.G., Defilpo, S.S. & Abril, A. 1999 Efecto de la adición de lectina específica sobre la simbiosis Rhizobium leguminosarum-Phaseolus vulgaris. Revista Argentina de Microbiologia 31, 72–77.
Vijayan, M. & Chandra, N. 1999 Lectins. Current Opinion in Strutural Biology 9, 707–714.
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Martínez, C.R., Albertini, A.V., Figueiredo, M.V. et al. Respiratory stimulus in Rhizobium sp. by legume lectins. World Journal of Microbiology and Biotechnology 20, 77–83 (2004). https://doi.org/10.1023/B:WIBI.0000013315.41026.cc
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DOI: https://doi.org/10.1023/B:WIBI.0000013315.41026.cc