Abstract
A bacterial strain LPM-410 capable of utilizing ethylenediaminetetraacetate (EDTA) as the sole source of energy, carbon, and nitrogen was isolated from sewage sludge and identified as a Pseudomonas sp. on the basis of its phenotypic characteristics. Suspensions of exponential-phase cells degraded EDTA, Mg–, Ca–, Ba–, and Mn–EDTA at constant specific rates ranging from 0.363 to 0.525 mmol EDTA/(g cells h). The more stable chelate, Zn–EDTA, was degraded at a lower rate (0.195 ± 0.030 mmol EDTA/(g cells h)), and here was no degradation of Co–, Cu–, Pb–, and Fe(III)–EDTA.
References
Bucheli-Witschel, M. & Egli, T. 2001 Environmental fate and microbial degradation of aminopolycarboxylic acids. FEMS Microbiology Reviews 25, 69–106.
DSM, Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH. (1993) Catalogue of Strains 1993. 5th edn., p. 356, Braunschweig: DSM. ISBN 0-683-00603-7.
Holt, J., Krieg, N., Seath, P., Staley, J. & Williams, S. 1994 Bergey's Manual of Determinative Bacteriology. 9th edn. Baltimore: Williams & Wilkins. ISBN 0-68300603-7.
Kluner, T., Hempel, D.C. & Nortemann, B. 1998 Metabolism of EDTA and its metal chelates by whole cells and cell-free extracts of strain BNC1. Applied Microbiology and Biotechnology 49, 194–201.
Lauff, J.J., Steele, D.B., Coogan, L.A. & Breitfeller, J.M. 1990 Degradation of the ferric chelate of EDTA by a pure culture of an Agrobacterium sp. Applied and Environmental Microbiology 56, 3346–3353.
Nortemann, B. 1992 Total degradation of EDTA by mixed cultures and a bacterial isolate. Applied and Environmental Microbiology 58, 671–676.
Satroutdinov, A.D., Dedyukhina, E.G., Chistyakova, T.I., Witschel, M., Eroshin, V.K. & Egli, T. 2000 Degradation of metal-EDTA complexes by resting cells of the bacterial strain DSM 9103. Environmental Science and Technology 34, 1715–1720.
Sillanpaa, M. 1997 Environmental fate of EDTA and DTPA. Reviews of Environmental Contamination and Toxicology 152, 85–111.
Smibert, R.M. & Krieg, N.R. 1994 Phenotypic characterization. In Methods for General and Molecular Bacteriology, eds. Gerhardt, P., Murray, R.G.E., Wood, W.A. & Krieg, N.R. pp. 607–654. Washington: American Chemical Society. ISBN 1-55581-048-9.
Witschel, M. 1999 Biochemical and physiological characterisation of a bacterial isolate able to grow with EDTA and other aminopolycarboxylic acids. PhD thesis, Swiss Federal Institute of Technology, Zurich.
Witschel, M., Weilenmann, H.-U. & Egli, T. 1995 Degradation of EDTA by a Bacterial Isolate. 54th Annual Meeting of the Swiss Society of Microbiology. Lugano.
Wolf, K. & Gilbert, P.A. 1992 EDTA Ethylenediaminetetraacetic acid. In The Handbook of Environmental Chemistry, ed. Hutzinger, O. Vol. 3, pp. 241–259. Berlin: Springer-Verlag. ISBN 3-54009690-6.
Zvyagintsev, M. 1991 Metody pochvennoi mikrobiologii i biokhimii (Methods of Soil Microbiology and Biochemistry), pp. 120–121. Moscow: Moscow State University (In Russian).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Chistyakova, T., Belikova, V., Satroutdinov, A. et al. A novel EDTA-degrading Pseudomonas sp.. World Journal of Microbiology and Biotechnology 19, 977–980 (2003). https://doi.org/10.1023/B:WIBI.0000007415.50506.3a
Issue Date:
DOI: https://doi.org/10.1023/B:WIBI.0000007415.50506.3a