Skip to main content
SpringerLink
Log in

Response of Pinus Sylvestris Seedlings to Cadmium and Mycorrhizal Colonisation

  • Published:
Water, Air, and Soil Pollution Aims and scope Submit manuscript

Abstract

Effects of Cd and mycorrhizal colonisation on seedling growth and Cd accumulation in Pinus sylvestris seedlings were studied. While colonisation by Suillus bovinus improved shoot growth, colonisation by Paxillus involutus had no effect on growth of seedlings. There was no Cd-ameliorating effect of colonisation in terms of either shoot or root growth. Colonisation by P. involutus did not decrease Cd transport from roots to shoots, whereas colonisation by S. bovinus significantly reduced the level of Cd in shoots in the 100 mg Cd kg-1 treatment. The proportion of healthy mycorrhizal root tips of S. bovinus-inoculated seedlings was reduced as a result of Cd addition. However, no such effect was found in P. involutus-inoculated seedlings.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ahonen-Jonnarth, U. and Finlay, R. D.: 2001, ‘Effects of elevated nickel and cadmium concentrations on growth and nutrient uptake of mycorrhizal and non-mycorrhizal Pinus sylvestris seedlings’, Plant Soil 236, 129–138.

    Article  CAS  Google Scholar 

  • Alloway, B. J.: 1995, Heavy Metals in Soils, 2nd ed., Blackie Academic and Professional, London.

    Google Scholar 

  • Alloway, B. J. and Steinnes, E.: 1999, ‘Anthropogenic additions of cadmium to soils’, in M. J. McLaughlin and B. R. Singh (eds), Cadmium in Soils and Plants, Kluwer Academic Publishers, Dordrecht, pp. 97–123.

    Google Scholar 

  • Balsberg Påhlsson, A.-M.: 1989, ‘Toxicity of heavy metals (Zn, Cu, Cd, Pb) to vascular plants’, Water, Air, Soil Pollut. 47, 287–319.

    Google Scholar 

  • Behrmann, P. and Heyser, W.: 1992, ‘Apoplastic transport through the fungal sheath of Pinus sylvestris/Suillus bovinus ectomycorrhizae’, Bot. Acta 105, 427–434.

    Google Scholar 

  • Boxman, A. W. and Roelofs, J. G. M.: 1988, ‘Some effects of nitrate versus ammonium nutrition on the nutrient fluxes in Pinus sylvestris seedlings. Effects of mycorrhizal infection’, Can. J. Bot. 66, 1091–1097.

    CAS  Google Scholar 

  • Brownlee, C., Duddridge, J. A., Malibari, A. and Read, D. J.: 1983, ‘The structure and function of mycelial systems of ectomycorrhizal roots with special reference to their role in forming interplant connections and providing pathways for assimilate and water transport’, Plant Soil 71, 433–443.

    Article  Google Scholar 

  • Brundrett, M. C., Piché, Y. and Peterson, R. L.: 1984, ‘A new method for observing the morphology of vesicular-arbuscular mycorrhizae’, Can. J. Bot. 62, 2128–2134.

    Google Scholar 

  • Bücking, H. and Heyser, W.: 1994, ‘The effect of ectomycorrhizal fungi on Zn uptake and distribution in seedlings of Pinus sylvestris L.’, Plant Soil 167, 203–212.

    Article  Google Scholar 

  • Chappelka, A. H., Kush, J. S., Runion, G. B., Meier, S. and Kelley, W. D.: 1991, ‘Effects of soilapplied lead on seedling growth and ectomycorrhizal colonization of loblolly pine’, Environ. Pollut. 72, 307–316.

    Article  CAS  Google Scholar 

  • Colpaert, J. V. and Van Assche, J. A.: 1992, ‘Zinc toxicity in ectomycorrhizal Pinus sylvestris’, Plant Soil 143, 201–211.

    Article  CAS  Google Scholar 

  • Colpaert, J. V., Van Assche, J. A. and Luijtens, K.: 1992, ‘The growth of the extramatrical mycelium of ectomycorrhizal fungi and the growth response of Pinus sylvestris L.’, New Phytol. 120, 127–135.

    Google Scholar 

  • Colpaert, J. V. and Van Assche, J. A.: 1993, ‘The effects of cadmium on ectomycorrhizal Pinus sylvestris L.’, New Phytol. 123, 325–333.

    CAS  Google Scholar 

  • Colpaert, J. V. and Vandenkoornhuyse, P.: 2001, ‘Mycorrhizal fungi’, in M. N. V. Prasad (ed.), Metals in the Environment: Analysis by Biodiversity, Marcel Dekker, New York, pp. 37–58.

    Google Scholar 

  • Coughtrey, P. J., Jones, C. H., Martin, M. H. and Sholes, S. W.: 1979, ‘Litter accumulation in woodlands contaminated by Pb, Zn, Cd and Cu’, Oecologia 39, 51–60.

    Article  Google Scholar 

  • Dixon, R. K.: 1988, ‘Response of ectomycorrhizal Quercus rubra to soil cadmium, nickel and lead’, Soil Biol. Biochem. 20, 555–559.

    Article  CAS  Google Scholar 

  • Dixon, R. K. and Buschena, C. A.: 1988, ‘Response of ectomycorrhizal Pinus banksiana and Picea glauca to heavy metals in soil’, Plant Soil 105, 265–271.

    CAS  Google Scholar 

  • Galli, U., Meier, M. and Brunold, C.: 1993, ‘Effects of cadmium on non-mycorrhizal and mycorrhizal Norway spruce seedlings [Picea abies (L.) Karst.] and its ectomycorrhizal fungus Laccaria laccata (Scop. ex Fr.) Bk. and Br.: sulphate reduction, thiols and distribution of the heavy metal’, New Phytol. 125, 837–843.

    CAS  Google Scholar 

  • Hartley, J., Cairney, J. W. G. and Meharg, A. A.: 1999, ‘Cross-colonization of Scots pine (Pinus sylvestris) seedlings by the ectomycorrhizal fungus Paxillus involutus in the presence of inhibitory levels of Cd and Zn’, New Phytol. 142, 141–149.

    Article  CAS  Google Scholar 

  • Hartley-Whitaker, J., Cairney, J. W. G. and Meharg, A. A.: 2000a, ‘Toxic effects of cadmium and zinc on ectomycorrhizal colonization of Scots pine (Pinus sylvestris L.) from soil inoculum’, Environ. Toxicol. Chem. 19, 694–699.

    Article  CAS  Google Scholar 

  • Hartley-Whitaker, J., Cairney, J.W. G. and Meharg, A. A.: 2000b, ‘Sensitivity to Cd or Zn of host and symbiont of ectomycorrhizal Pinus sylvestris L. (Scots pine) seedlings’, Plant Soil 218, 31–42.

    Article  CAS  Google Scholar 

  • Hüttermann, A., Arduini, I. and Godbold, D. L.: 1999, ‘Metal pollution and forest decline’, in M. N. V. Prasad and J. Hagemeyer (eds), Heavy Metal Stress in Plants: From Molecules to Ecosystems, Springer, Berlin, pp. 253–272.

    Google Scholar 

  • Jentschke, G. and Godbold, D. L.: 2000, ‘Metal toxicity and ectomycorrhizas’, Physiol. Plant. 109, 107–116.

    Article  CAS  Google Scholar 

  • Jentschke, G., Winter, S. and Godbold, D. L.: 1999, ‘Ectomycorrhizas and cadmium toxicity in Norway spruce seedlings’, Tree Physiol. 19, 23–30.

    CAS  Google Scholar 

  • Jones, M. D. and Hutchinson, T. C.: 1988, ‘Nickel toxicity in mycorrhizal birch seedlings infected with Lactarius rufus or Scleroderma flavidum. I. Effects on growth, photosynthesis, respiration and transpiration’, New Phytol. 108, 451–459.

    CAS  Google Scholar 

  • Kim, C.-G., Power, S. A. and Bell, J. N. B.: 2003a, ‘Effects of Cd on growth and glucose utilisation of ectomycorrhizal fungi in vitro’, Mycorrhiza 13, 223–226.

    Article  CAS  Google Scholar 

  • Kim, C.-G., Bell, J. N. B. and Power, S. A.: 2003b, ‘Effects of soil cadmium on Pinus sylvestris L. seedlings’, Plant Soil 257, 443–449.

  • Leyval, C., Turnau, K. and Haselwandter, K.: 1997, ‘Effect of heavy metal pollution on mycorrhizal colonization and function: physiological, ecological and applied aspects’, Mycorrhiza 7, 139–153.

    Article  CAS  Google Scholar 

  • Marx, D. H.: 1969, ‘The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria’, Phytopathology 59, 153–163.

    Google Scholar 

  • Marx, D. H. and Zak, B.: 1965, ‘Effect of pH on mycorrhizal formation of slash pine in aseptic culture’, For. Sci. 11, 66–75.

    Google Scholar 

  • Marx, D. H., Bryan, W. C. and Davey, C. B.: 1970, ‘Influence of temperature on aseptic synthesis of ectomycorrhizae by Thelephora terrestris and Pisolithus tinctorius on loblolly pine’, For. Sci. 16, 424–431.

    Google Scholar 

  • Meharg, A. A. and Cairney, J. W. G.: 2000, ‘Co-evolution of mycorrhizal symbionts and their hosts to metal-contaminated environments’, Adv. Ecol. Res. 30, 69–112.

    CAS  Google Scholar 

  • Pöder, R.: 1996, ‘Ectomycorrhizae’, in F. Schinner, R. Öhlinger, E. Kandeler and R. Margesin (eds), Methods in Soil Biology, Springer, Berlin, pp. 281–294.

    Google Scholar 

  • Rouhier, H. and Read, D. J.: 1998, ‘Plant and fungal responses to elevated atmospheric carbon dioxide in mycorrhizal seedlings of Pinus sylvestris’, Environ. Exp. Bot. 40, 237–246.

    Article  Google Scholar 

  • Simard, S. W., Perry, D. A., Jones, M. D., Myrold, D. D., Durall, D. M. and Molina, R.: 1997, ‘Net transfer of carbon between ectomycorrhizal tree species in the field’, Nature 388, 579–582.

    Article  CAS  Google Scholar 

  • Smith, S. E. and Read, D. J.: 1997, Mycorrhizal Symbiosis, 2nd ed. Academic Press, San Diego.

    Google Scholar 

  • Tennant, D.: 1975, ‘A test of a modified line intersect method of estimating root length’, J. Ecol. 63, 995–1001.

    Google Scholar 

  • Turnau, K., Kottke, I., Dexheimer, J. and Botton, B.: 1994, ‘Element distribution in mycelium of Pisolithus arrhizus treated with cadmium dust’, Ann. Bot. 74, 137–142.

    Article  CAS  Google Scholar 

  • Van Tichelen, K. K., Vanstraelen, T. and Colpaert, J. V.: 1999, ‘Nutrient uptake by intact mycorrhizal Pinus sylvestris seedlings: a diagnostic tool to detect copper toxicity’, Tree Physiol. 19, 189–196.

    CAS  Google Scholar 

  • Van Tichelen, K. K., Colpaert, J. V. and Vangronsveld, J.: 2001, ‘Ectomycorrhizal protection of Pinus sylvestris against copper toxicity’, New Phytol. 150, 203–213.

    Article  CAS  Google Scholar 

  • Wilkins, D. A.: 1991, ‘The influence of sheathing (ecto-) mycorrhizas of trees on the uptake and toxicity of metals’, Agric. Ecosyst. Environ. 35, 245–260.

    CAS  Google Scholar 

  • Wilson, M. J. and Bell, N.: 1996, ‘Acid deposition and heavy metal mobilization’, Appl. Geochem. 11, 133–137.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Environmental Science and Technology, Imperial College London, Silwood Park Campus, Ascot, Berkshire, SL5 7PY, UK

    C.-G. Kim, S. A. Power & J. N. B. Bell

Authors
  1. C.-G. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. A. Power
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. N. B. Bell
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. N. B. Bell.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, CG., Power, S.A. & Bell, J.N.B. Response of Pinus Sylvestris Seedlings to Cadmium and Mycorrhizal Colonisation. Water, Air, & Soil Pollution 155, 189–203 (2004). https://doi.org/10.1023/B:WATE.0000026527.34649.3c

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:WATE.0000026527.34649.3c

Search

Navigation