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Rinderpest Virus RNA Polymerase Subunits: Mapping of Mutual Interacting Domains on the Large Protein L and Phosphoprotein P

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Abstract

The RNA dependant RNA polymerase of negative sense RNA viruses is composed of two subunits – the Large protein (L) and the Phosphoprotein (P). These two proteins have to form a complex in order to carry out genome transcription and replication. Employing the baculovirus expression system, we demonstrate here, the specific in vivo interaction between the L and P proteins of Rinderpest virus and also the stabilization of L protein when it is present as L + P complex. The regions on either protein involved in such interaction has been studied using the yeast two-hybrid system which indicates that the P binding region resides within the amino terminal 380 amino acid residues of L protein. The L binding region on P protein has been mapped to lie within 347–490 amino acids.

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Authors and Affiliations

  1. Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, 200 Lothrop Street, Pittsburgh, PA, 15213, USA

    Anasuya Chattopadhyay

  2. Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560 012, India

    Anasuya Chattopadhyay & M.S. Shaila

Authors
  1. Anasuya Chattopadhyay
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  2. M.S. Shaila
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Correspondence to M.S. Shaila.

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Chattopadhyay, A., Shaila, M. Rinderpest Virus RNA Polymerase Subunits: Mapping of Mutual Interacting Domains on the Large Protein L and Phosphoprotein P. Virus Genes 28, 169–178 (2004). https://doi.org/10.1023/B:VIRU.0000016855.25662.95

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  • DOI: https://doi.org/10.1023/B:VIRU.0000016855.25662.95

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