Abstract
There is resurgence of interest in the study of occurrence, genotype and pathogenic associations of human Polyomavirus BK and JC in recent years. In the present study, we have ascertained the presence of BK virus shed in the urine samples of pregnant women and immunocompromised patients, for the first time in Asian Indian population, and have also characterised the prevalent genotypes of the non-coding control regions (NCCRs) of these natural isolates. The results strongly suggest a very high incidence, as well as degree, of BK virus reactivation in this population groups assayed. Approximately 65% of the patients and pregnant women together, tested positive based on polymerase chain reaction (PCR) analysis, and these results were further confirmed by Southern hybridisation and dot blot against BKV specific probes. The NCCRs of the several Indian endemic strains were analysed by sequencing PCR products, amplified directly from urine samples, with oligonucleotide primers designed from the constant region of T-Antigen and VP2 coding sequences. The typical features of the NCCRs of these Indian strains appeared to be comparable and related to the archetypal strain BKV (WW) with some alterations in few key positions. Apart from these subtle alterations, neither any major DNA rearrangement within the NCCR region nor any drastic modification marked BKV strains found in nephropathy and in the healthy subjects (pregnancy). However, in some of the immunocompromised patients studied, the degree of reactivations reflected by viruria, appeared to be much higher compared to other reports.
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References
Gardner S.D., Part I., Br Med J 77–78, 1973.
Shah K.V., Daniel R.W., and Warszawski R.M., J Infect Dis 128, 784–787, 1973.
Brown P., Tsai T., and Gajdusek D.C., Am J Epidemiol 102, 331–340, 1975.
Greenlee J.E., in Richman D.D., Whitley R.J., and Hayden F.G. (eds), Polyomavirus (Chapter 26), Clinical Virology, Churchill Livingstone Inc., New York, 1997, pp. 549–567.
Chesters P.M., Heritage J., and McCance D.J., J Infect Dis 147, 676–684, 1983.
Markowitz R.B., Eaton B.A., Kubic M.F., Latorra D., McGregor J.A., and Dynan W.S., J Virol 65, 4515–4519, 1991.
Gardner S.D., Field A.M., Coleman D.V., and Hulme B., Lancet I, 1253–1257, 1971.
Rosen S., Harmon W., Krensky A., Edelson P.J., Padgett B.J., Grinnell B.W., Rubino M.J., and Walker D.L., N Eng J Med 308, 1192–1196, 1983.
Gardner S.D., Mackenzie E.F.D., Smith C., and Porter A.A., J Clin Path 37, 578–586, 1984.
Arthur R.R., Shah K.V., Baust S.J., Santos G.W., and Saral R., N Eng J Med 315, 230–234, 1986.
Smith R.D., Galla J.H., Skahan K., Anderson P., Linnemann C.C., Ault G.S., Ryschkewitsch C.F., and Stoner G.L., J Clin Microbiol 36, 1660–1665, 1998.
Geetha D., Tong B.C., Racusen L., Markowwitz J.S., and Westra W.H., Transplantation 73, 1933–1936, 2002.
Neel J.V., Major E.G., Awa A.A., Glover T., Burgess A., Traub R., Curfinan B., and Satoh C., Proc Natl Acad Sci USA 93, 2690–2695, 1996.
Neel J.V., Am J Hum Genet 63, 489–497, 1998.
Laghi L., Randolph A.E., Chauhan D.P., Marra G., Major E.O., Neel J.V., and Boland R.C., Proc Natl Acad Sci USA 96, 7484–7489, 1999.
Markowitz R.B., Thompson H.C., Mueller J.F., Cohen J.A., and Dynan W.S., J Infect Dis 167, 13–20, 1993.
Moens U., Johansen T., Johnsen J.I., Seternes O.M., and Traavik T., Virus Genes 10, 261–275, 1995.
Seif I., Khoury G., and Dhar R., Cell 18, 963–977, 1979.
Deyerle K.L., Cassill A.J., and Subramani S., Virology 158, 181–193, 1987.
Deyerle K.L. and Subramani S., J Virol 62, 3378–3387, 1988.
Chakraborty T. and Das G.C., Mol Cell Biol 9, 3821–3828, 1989.
Flaegstad T., Nilsen I., Skar A.G., and Traavik T., Scand J Infect Dis 23, 287–291, 1991.
Negrini M., Sabbioni S., Arthur R.R., Castagnoli A., and Barbanti-Brodano G., J Virol 65, 5092–5095, 1991.
Chang D., Tsai R., Wang M., and Ou W., J Med Virol 48, 204–209, 1996.
Southern E.M., J Mol Biol 98, 503–517, 1975.
Ausubel F.M. (ed). Current Protocols in Molecular Biology. vol. 1, Unit 6.4, John Wiley and Sons Inc., 1995.
Sundsfjord A., Johansen T., Flaegstad., Moens U., Villard P., Subramani S., and Traavik T., J Virol 64, 3864–3871, 1990.
Kitamura T., Yogo Y., Kunitake T., Suzuki K., Tajima A., and Kawabe K., Intl J Urol 1, 28–32, 1994.
Jin L., Gibson P.E., Booth J.C., and Clewly J.P., J Med Virol 41, 11–17, 1993.
Kitamura T., Aso Y., Kuniyoshi N., Hara K., and Yogo Y., J Infect Dis 161, 1128–1133, 1990.
Deb S., Tsui S., Koff A., DeLucia A.L., Parsons R., and Tegtmeyer P., J Virol 61, 2143–2149, 1987.
Mew R.T., Lecatsas G., Prozesky O.W., and Harley O.H., Intervirology 16, 14–19, 1981.
Rubinstein R., Pare N., and Harley E.H., J Virol 61, 1747–1750, 1987.
Chakraborty T. and Das G.C., J Gen Virol 72, 1935–1942, 1991.
Moens U., Subramaniam N., Johansen B., Johansen T., and Traavik T., J Virol 68, 2398–2408, 1994.
Tada H., Rappaport J., Lashgari M., Amini S., Wong-Staal F., and Khalili K., Proc Natl Acad Sci USA 87, 3479–3483, 1990.
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Bhattacharjee, S., Chakraborty, T. High Reactivation of BK Virus Variants in Asian Indians with Renal Disorders and During Pregnancy. Virus Genes 28, 157–168 (2004). https://doi.org/10.1023/B:VIRU.0000016854.37475.f3
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DOI: https://doi.org/10.1023/B:VIRU.0000016854.37475.f3