Abstract
Ovulation in the Bactrian camel depends upon ovulation-inducing factors in the seminal plasma. The present study was conducted to isolate and purify the bioactive fractions from the seminal plasma of these camels. The seminal plasma was fractionated by anion-exchange chromatography, and six fractions were obtained. The bioactive potential of each fraction was estimated from its effect on rat pituitary tissue culturedin vitro and by the effect of an intramuscular injection of the fraction into female camelsin vivo. Both the third fraction (F3) and the fifth fraction (F5) stimulated the release of LHin vitro andin vivo. In addition, female camels ovulated within 48 h after intramuscular injection of F3. However, neither F3 nor F5 had any significant effect on the secretion of FSH, eitherin vitro orin vivo. When F3 was further fractionated into four subfractions, the third subfraction (F3-3) still stimulated the in vitro release of LH, but not of FSH. An attempt to further purify the ovulation-inducing factors in F3-3 failed owing to the similarity of the molecular characters.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Al Somai, N., Vishwanath, R., Molan, P.C. and Shannon, P., 1994a. Anionic and cationic components from protein aggregates in bovine seminal plasma and their effects on sperm motility. Molecular Reproductive Development, 39, 328-336
Al Somai, N., Vishwanath, R., Shannon, P. and Molan, P.C., 1994b. Low molecular weight components in bovine semen diffusate and their effects on motility of bull sperm. Reproduction and Fertility Development, 6, 165-171
Chen, B.X., Kang, C.L., Yuan, Z.X. and Ge, R.X., 1980. Reproductive pattern of the Bactrian camel. II The sexual activities of the camel. Acta Veterinaria et Zootechnica Sinica, 11, 65-76
Chen, B.X., Yuan, Z.X., Huang, Y.M. and Pan, G.W., 1985. Semen induced ovulation in the Bactrian camel. Journal of Reproduction and Fertility, 74, 335-339
Einspanier, R., Einspanier, A., Wempe, F. and Schekit, K.H., 1991. Characterization of a new bioactive protein from bovine seminal fluid. Biochemical and Biophysical Research Communications, 179, 1006-1010
Lavon, U., 1972. Characterization of boar, bull, ram and rabbit seminal proteins by gel disc electrophoresis and isoelectric focusing on polyacrylamide. Journal of Reproduction and Fertility, 31, 29-37
Saacke, R.G., Dalton, J.C., Nadir, S., Nebel, R.L. and Bame, J.H., 2000. Relationship of seminal traits and insemination time to fertility and embryo quality. Animal Reproduction Science, 60-61, 663-667
Smith, M.L., 1985. Release of luteinizing hormone and follicle stimulating hormone from pituitary of lactating rats by gonadotropin-releasing hormone and high potassium concentration. Endocrinology, 116, 1826-1834
Wempe, F., Einspanier, R. and Scheit, K.H., 1992. Characterization by cDNA cloning of the mRNA of a new growth factor from bovine seminal plasma: acidic seminal plasma fluid protein. Biochemical and Biophysical Research Communications, 183, 232-237
Xu, Y.S., Wang, H.Y., Zeng, G.Q., Jiang, G.T. and Gao, Y.H., 1985. Hormone concentrations before and after semen-induced ovulation in the Bactrian camel (Camelus bactrianus). Journal of Reproduction and Fertility, 74, 341-346
Zhao, X.X., Pan, G.W. and Huang, Y.M., 1990. Studies on the ovulation-inducing factors in the seminal plasma of Bactrian camel. In: Saint-Martin G (ed.), Proceedings of the Workshop Is it Possible to Improve the Reproductive Performance of the Camel? (CIRAD-EMVT, Paris), 197-208
Zhao, X.X., Huang, Y.M. and Chen, B.X., 1992. Biological activity of gonadotrophin-releasing hormone-like factors in the seminal plasma of the Bactrian camel (Camelus bactrianus). In: Allen, W.R., Higgins, A.J., Mayhew, I.G., Snow, D.H. and Wade, J.F. (eds), Proceedings of the First International Camel Conference, UK, (R and W Publications, Newmarket), 163-168
Zhao, X.X., Huang, Y.M., Nic, Q.C., Zhang, Y.K. and Chen B.X., 1996. Effects of different extenders on motility, survival and acrosomal integrity of camel spermatozoa frozen in ampoules. Journal of Camel Practice Research, 6, 23-26
Zhao, X.X., Li, X.L. and Chen, B.X., 2001. Isolation of ovulation-inducing factors in the seminal plasma of Bactrian camel (Camelus bactrianus) by DEAE-cellulose chromatography. Reproduction in Domestic Animals, 36, 177-181
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Li, X., Zhao, X. Separation and Purification of Ovulation-inducing Factors in the Seminal Plasma of the Bactrian Camel (Camelus bactrianus). Vet Res Commun 28, 235–245 (2004). https://doi.org/10.1023/B:VERC.0000017370.74401.be
Issue Date:
DOI: https://doi.org/10.1023/B:VERC.0000017370.74401.be