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A Serological Investigation into Contagious Caprine Pleuropneumonia (CCPP) in Ethiopia

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Abstract

For a comparison of serological tests for CCPP, sera from 767 goats were examined. They were subjected to three tests: complement fixation test (CFT) with Mycoplasma capricolum subspecies capripneumoniae antigen; blocking ELISA (B-ELISA) with Mycoplasma capricolum subspecies capripneumoniae antigen; and CFT with Mycoplasma mycoides subspecies mycoides small colony type antigen. Antibodies were detected by these three tests in 23%, 2% and 12%, respectively, of sera from districts in which CCPP had not been reported, and in 60%, 83% and 87%, respectively, in sera from areas in which CCPP had been reported. The specificity of the tests is discussed. The use of B-ELISA test for the diagnosis and for epidemiological studies of CCPP is strongly recommended.

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REFERENCES

  • Bashiruddin, J.B., Taylor, T.K. and Gould, A.R., 1994. A PCR-based test for the specific identification of Mycoplasma mycoides subspecies mycoides SC. Journal of Veterinary Diagnostic Investigations, 6, 428–434

    Google Scholar 

  • Bonnet, F., Saillard, C., Bove, J.M., Leach, R.H.L., David, R., Cottew, G.S. and Tully, J.G., 1993. DNA relatedness between field isolates of Mycoplasma F38 group, the agent of contagious caprine pleuropneumonia, and strains of Mycoplasma capricolum. International Journal of Systematic Bacteriology, 43, 597–602

    Google Scholar 

  • Campbell, A.D. and Turner, A.W., 1953. Reference for CFT antigen. Australian Veterinary Journal, 29 , 154

    Google Scholar 

  • Cottew, G.S., 1979. Pathogenicity of the subspecies mycoides of Mycoplasna mycoides for cattle, sheep and goats. Zentralblatt fur Bakteriologie [Orig A], 245, 164–170

    Google Scholar 

  • Daniel, B.M., Stuart, J. and Edelstein, S.A.J., 1991. Protein concentration determination. Protein Methods, pp. 45–55

  • Gourlay, R.N., 1964. Modified Newing's tryptose broth. Research in Veterinary Science, 5, 473–482

    Google Scholar 

  • GTZ-BgVV Service Laboratory, 1998. Complement fixation test. Supraregional Project on Adapted Diagnostic Technique in Veterinary Medicine, 1–3

  • Kusiluka, L.J., Ojeniyi, B., Friis, N.F., Kokotovic, B. and Ahrens, P., 2001. Molecular analysis of field strains of Mycoplasma capricolum subspecies capripneumoniae and Mycoplasma mycoides subspecies mycoides, small colony type isolated from goats in Tanzania. Veterinary Microbiology, 82, 27–37

    Google Scholar 

  • OIE, 1996. Contagious caprine pleuropneumonia. Manual of Standards for Diagnostic Tests and Vaccines, (Office International des Epizooties), 380

  • Rurangirwa, FR., McGuire, T.C. and Magnuson, N.S., 1987. Composition of a polysaccharide from Mycoplasma F38 recognised by antibodies from goats with contagious pleuropneumonia. Research in Veterinary Science, 42, 175–178

    Google Scholar 

  • Rurangirwa, F.R., Kouyate, B., Niang, M. and McGuire, T.C., 1990. Contagious caprine pleuropneumonia antibodies to F38 polysaccharides in Mali goats. The Veterinary Record, 127, 353

    Google Scholar 

  • Seifert, H.S.H., 1996. Contagious caprine pleuropneumonia. Tropical Animal Health, 2nd edn, (Kluwer Academic Publishers, Dordrecht), 339-341

    Google Scholar 

  • Thiaucourt, F., Breard, A., Lefevre, P.C. and Mebratu, G.Y., 1992. Contagious caprine pleuropneumonia in Ethiopia. The Veterinary Record, 131, 585

    Google Scholar 

  • Thiaucourt, F., Libeau, G., LeGoff, C. and Lefevre, P.C., 1994. The use of Mab in the diagnosis of contagious caprine pleuropneumonia. Veterinary Microbiology, 41, 191–203

    Google Scholar 

  • Thiaucourt, F., Lorenzon, S., David, A. and Breard, A., 2000. Phylogeny of the Mycoplasma mycoides cluster as shown by sequencing of a putative membrane protein gene. Veterinary Microbiology, 72, 251–268

    Google Scholar 

  • Wamwayi, H.M., Wafula, J.S., Litamoi, J.K. and Nandokha, E., 1989. Detection of antibody to Mycoplasma F-38 in goats sera by an ELISA. Tropical Animal Health and Production, 21, 43–49

    Google Scholar 

  • Wesonga, H.O., Litamio, J.K., Kagumba, M. and Wakhusama, E., 1993. Relationship between clinical signs and early lesions of contagious caprine pleuropneumonia caused by Mycoplasma strain F-38. Small Ruminant Research, 10, 45–54

    Google Scholar 

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Authors and Affiliations

  1. Ministry of Agriculture, National Veterinary Institute, Debre Zeit, Ethiopia

    A.D. Sharew

  2. GTZ-BgVV Service Laboratory, Berlin, Germany

    C. Staak

  3. CIRAD-EMVT, Montpellier, France

    F. Thiaucourt & F. Roger

Authors
  1. A.D. Sharew
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  2. C. Staak
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  3. F. Thiaucourt
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  4. F. Roger
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Sharew, A., Staak, C., Thiaucourt, F. et al. A Serological Investigation into Contagious Caprine Pleuropneumonia (CCPP) in Ethiopia. Tropical Animal Health and Production 37, 11–19 (2005). https://doi.org/10.1023/B:TROP.0000047921.19064.d9

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  • DOI: https://doi.org/10.1023/B:TROP.0000047921.19064.d9

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