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Plant Cell, Tissue and Organ Culture

, Volume 79, Issue 1, pp 87–90 | Cite as

Age and Physiological Condition of Donor Plants Affect in vitro Morphogenesis in Leaf Explants of Passiflora edulis f. flavicarpa

  • D.C. Becerra
  • A.P. Forero
  • G.A. Góngora
Article

Abstract

A protocol for in vitro shoot regeneration from leaf discs of Passiflora edulis f. flavicarpa is described. The effect of explant source on morphogenesis was studied by comparing the response of explants collected from young plants, in vitro plantlets, and reinvigorated adult plants. Highest de novo shoot production was observed in explants from 2-month-old plants cultured in Murashige and Skoog medium supplemented with sucrose 30 g l−1, 4.44 µM benzyladenine, and 2.32 µM kinetin. Regenerated shoots rooted spontaneously in growth regulator-free medium and were successfully acclimatized to greenhouse conditions. Influence of the age and physiological state on shoot regeneration and gradual loss of morphogenic capacity is discussed.

in vitro plant regeneration maracuya organogenesis yellow passion fruit 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • D.C. Becerra
    • 1
  • A.P. Forero
    • 1
  • G.A. Góngora
    • 1
  1. 1.Unidad de Biotecnología Vegetal, Departamento de BiologíaUniversidad JaverianaBogotáColombia

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