Abstract
Mulberry (Morus alba L.) is a dioecious plant. In vitro sex modification in mulberry by using two chemicals, viz., ethrel and silver nitrate, is described. 2-Chloroethylphosphonic acid (ethrel) and silver nitrate were filter sterilized and added to Murashige and Skoog (1962) medium supplemented with 5 µM 6-benzyl amino purine (BA). Ethrel applied at different concentrations produced female, male and mixed inflorescences in male mulberry nodal cuttings. Maximum 13.6% female inflorescences were observed on media containing 2000 µg l−1 ethrel. The induction of male, female and mixed inflorescences was observed in female plants after applying silver nitrate to the culture medium. The number of male inflorescences increased with the silver nitrate concentration to a maximum of 22.4% induction on 2500 µg l−1. Bisexual flowers were also observed along with male and female flowers in silver nitrate treated plants.
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References
Anonymous (1975) Textbook of Tropical Sericulture. Japan Overseas Cooperation Volunteers, 4-2-4 Hiroo Sibuya-Ku, Tokyo, Japan
Atsmon D & Tabbak C (1979) Comparative effects of gibberellin, silver nitrate and aminoethoxyvinyl glycine on sexual tendency and ethylene evolution in the cucumber plant (Cucumis sativus L.) Plant Cell Physiol. 20: 1547–1555
Beyer E (1976) Silver ion: a potent antiethylene agent in cucumber and tomato. Hort. Sci. 11: 195–196
Camerarius (1964) Taxonomy of Flowering Plants. CL Porter, Freeman, San Francisco (p. 13)
Ghaemi M, Sarrafi A & Alibert G (1994) The effects of silver nitrate, colchicines, cupric sulfate and genotype on the production of embryoids from anthers of tetraploid wheat (Triticum turgidum). Plant Cell Tiss. Org. Cult. 36: 355–359
Johansen DA (1940) Plant Microtechnique. McGraw-Hill, New York
Lower RL, Pharr DM & Horst EK (1978) Effects of silver nitrate and gibberellic acid on gynoecious cucumber. Cucur. Gen. Coop. Rep. 1: 8–9
Mohan Ram HY & Jaiswal VS (1970) Induction of female flowers on male plants of Cannabis sativa L. by 2-chloroethanephosphonic acid. Experientia 26: 214–216
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497
Narayan P, Raghunath MK, Chakroborthi SP & Sen SK (1993) In vitro flowering and fertilization for mulberry breeding. Ind. Silk. 13: 45–46
Ockendon DJ & Clenagham R (1993) Effect of silver nitrate and 2,4-D on anther culture of Brussels sprouts. Plant Cell Tiss. Org. Cult. 32: 41–46
Oka S & Ohyama K (1986) Mulberry (Morus alba L). In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, Vol. 1. Trees (pp. 384–392). Springer-Verlag, Berlin
Rudich J, Halevy AH & Kedar N (1972) Ethylene evolution from cucumber plants as related to sex expression. Plant Physiol. 49: 998–999
Saito T & Takahashi H (1987) Role of leaves in ethylene induced femaleness in cucumber plants. J. Jpn. Soc. Hort. Sci. 55: 445–454
Stankovic L & Prodanovic S (2002) Silver nitrate effects on sex expression in cucumber (Cucumis sativus). II Balkan Symposium on Vegetables and Potatoes. ISHS Acta Hort. 579
Takahashi H & Jaffe MJ (1984) Further studies of auxin and ACC induced feminization in cucumber plant using ethylene inhibitors. Phyton 44: 81–86
Trebitsh T, Riov J & Rudich J (1987) Auxin, biosynthesis of ethylene and sex expression in cucumber (Cucumis sativus). Plant Growth Regul. 5: 105–113
Yin T & Quinn JA (1995) Tests of a mechanistic model of one hormone regulating both sexes in Cucumis sativus (Cucurbitaceae). Am. J. Bot. 82: 1537–1546
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Dennis Thomas, T. In Vitro Modification of Sex Expression in Mulberry (Morus Alba) by Ethrel and Silver Nitrate. Plant Cell, Tissue and Organ Culture 77, 277–281 (2004). https://doi.org/10.1023/B:TICU.0000018390.65934.93
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DOI: https://doi.org/10.1023/B:TICU.0000018390.65934.93