Abstract
The effects of sucrose concentration (1, 3, 5, or 7% w/v) in liquid media, in the presence and absence of benzylaminopurine (BAP), on internal carbohydrate status and growth of Hosta tokudama Tratt. ‘Newberry Gold’ during the multiplication phase (stage II) was investigated. Cultures from all treatment combinations were transferred to media containing 3% (w/v) sucrose during the rooting phase (stage III). At the end of the stage III, these micropropagules were subjected to 5 weeks of storage at 10 °C under low light (photosynthetic photon flux of 5 µmol m−2s−1). Endogenous concentrations of soluble sugars (glucose, fructose, and sucrose) in the plantlets increased linearly as the media sucrose concentration increased from 1% to 7% during stage II. Root and shoot biomass increased with increasing media sucrose concentration. BAP increased the biomass and multiplication rate but did not affect internal concentration of soluble sugars. While in storage, endogenous sugar levels and plantlet dry weight remained unchanged for all treatments. Following storage, plants originally cultured in 5% and 7% media sucrose had higher dry weight and less leaf chlorosis than those cultured in 1% and 3% media. Differences in endogenous soluble sugar levels at the end of stage III rooting, and after storage were related to the sucrose concentration of the initial stage II multiplication medium. Increased media sucrose levels during the multiplication cycle has a positive, long-term effect on plant morphology and quality.
Similar content being viewed by others
References
Adelberg J, Kroggel M & Toler J (2000) Physical environment in vitro affects laboratory and nursery growth of micropropagated Hostas. HortTechnology 10: 754–757
Amor Y, Haigler CH, Wainscott M, Johnson S & Delmer DP (1995) A membrane-associated form of sucrose synthase and its potential role synthesis of cellulose and callose in plants. Proc. Natl. Acad. Sci. USA 92: 9353–9357
Armitage A (1997) Herbaceous perennial plants (pp. 537–540). Stipes Publishing, LLC. Champaign IL USA
Babb VM & Candace Haigler H (2001) Sucrose phosphate synthase activity rises in correlation with high-rate cellulose synthesis in three heterotrophic systems. Plant Physiol. 127: 1234–1242
Bajaj YPS (1986) In vitro preservation of genetic resources – techniques and problems. Int Symp Nuclear Techniques and in vitro Culture for Plant Improvement (pp. 43–57). IAEA/FAO, Vienna
Bajaj YPS (1991) Storage and cryopreservation of in vitro cultures. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry Vol 17. Hi-Tech and Micropropagation I (pp. 361–381). Springer, Berlin Heidelberg
Capellades M, Lemeur R & Debergh P (1990) Effects of sucrose on starch accumulation and rate of photosynthesis in Rosa cultured in vitro. Plant Cell Tiss. Org. Cult. 25: 21–26
Chu I (1995) Economic analysis of automated micropropagation. In: Aitken Christie J, Kozai T & Smith MAL (eds) Automation and Environmental Control in Plant Tissue Culture (pp. 19–28). Kluwer Academic Publishers, Dordecht, The Netherlands
D'Agostino IB & Kieber JJ (1999) Molecular mechanisms of cytokinin action. Curr. Opin. Plant Biol. 2: 359–364
Etienne H & Berthouly M (2002) Temporary immersion systems in plant micropropagation. Plant Cell Tiss. Org. Cult. 69: 215–231
Haissig BE & Dickson RE (1979) Starch measurements in plant tissue using enzymatic hydrolysis. Physiol. Plant. 47: 151–157
Kubota C & Kozai T (1994) Low-temperature storage for quality preservation and growth suppression of broccoli seedlings cultured in vitro. HortScience. 29: 1191–1194
Miller WB & Langhans RW (1989) Carbohydrate changes of Easter lilies during growth in normal and reduced irradiance environments. J. Am. Soc. Hort. Sci. 114: 310–315
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Smith MAL & Spomer LA (1995) Vessels, gels, liquid media and support systems. In: Aitken-Christie J, Kozai T & MAL Smith (eds) Automation and Environmental Control in Plant Tissue Culture (pp. 371–404). Kluwer Academic Publishers, Dordrecht, The Netherlands
Wilson SB, Rajapakse NC & Young RE (1998) Low temperature storage of micropropagated Hosta plantlets under various light qualities. Proc. Southern Nursery Assoc. Res. Conf. 43: 320–323
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gollagunta, V., Adelberg, J.W., Rieck, J. et al. Sucrose Concentration in Liquid Media Affects Soluble Carbohydrates, Biomass and Storage Quality of Micropropagated Hosta. Plant Cell, Tissue and Organ Culture 77, 125–131 (2004). https://doi.org/10.1023/B:TICU.0000016814.88023.22
Issue Date:
DOI: https://doi.org/10.1023/B:TICU.0000016814.88023.22