Abstract
The G258 mutant cell line, isolated from the FM3A mouse mammary carcinoma cell line, is temperature-sensitive for both cell growth and asparagine-linked glycosylation due to mutation at a single location. The biochemical defect in the G258 mutant resides in the formation of lipid-linked oligosaccharide, presumably in one of the steps of GDP-mannose-dependent mannosylation (Y. Nishikawa, J. Cell. Physiol. 119, 260–266, 1984; Y. Nishikawa, Biochim. Biophys. Acta 1091, 135–140, 1991). In the present study, we transfected human genomic DNA fragments into the G258 mutant by the radiation hybrid method and isolated transformants (KK-1, -3 and -4) which showed recovery from both temperature-sensitive cell growth and asparagine-linked glycosylation. These transformants contained a common Alu-containing human DNA fragment (1.3 kb) which will be used as a marker for isolating the gene that complements the defect of lipid-linked oligosaccharide synthesis in the G258 mutant.
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Kataoka, K., Takahashi, T., Ayusawa, D. et al. Characterization of a Human Genomic DNA Fragment Which Rescues Defective Lipid-Linked Oligosaccharide Synthesis in a Mutant G258 Cell Line Isolated from the FM3A Mouse Mammary Carcinoma Cell Line. Somat Cell Mol Genet 24, 235–243 (1998). https://doi.org/10.1023/B:SCAM.0000007125.41715.8d
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DOI: https://doi.org/10.1023/B:SCAM.0000007125.41715.8d