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Russian Journal of Plant Physiology

, Volume 51, Issue 6, pp 806–814 | Cite as

Compartmentation of α- and β-Carbonic Anhydrases in Cells of Halo- and Alkalophilic Cyanobacteria Rhabdoderma lineare

  • M. V. Dudoladova
  • A. G. Markelova
  • N. V. Lebedeva
  • N. A. Pronina
Article

Abstract

The organization of carbonic anhydrase (CA) system in halo- and alkalophilic cyanobacteria Rhabdoderma lineare was studied by Western blot analysis and immunocytochemical electron microscopy. The presence of extracellular α-CA (60 kD) in the glycocalyx, forming a tight sheath around the cell, and of two intracellular β-CA is reported. One β-CA (60 kD) is associated with polypeptides of photosystem II (PSII) and is a constitutive enzyme. Another β-carbonic anhydrase (25 kD) was induced by low content of bicarbonate in the culture medium; this inducible CA was found in the fraction of total soluble proteins. The expressed synthesis of inducible β-CA was accompanied by the increase in the intracellular pool of inorganic carbon, which suggests an important role of this enzyme in the functioning of CO2-concentrating mechanism.

Rhabdoderma lineare alkalophilic cyanobacteria of soda lakes α- and β-carbonic anhydrases photosystem II thylakoid membranes CO2-concentrating mechanism 

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Copyright information

© MAIK “Nauka/Interperiodica” 2004

Authors and Affiliations

  • M. V. Dudoladova
    • 1
  • A. G. Markelova
    • 1
  • N. V. Lebedeva
    • 1
  • N. A. Pronina
    • 1
  1. 1.Timiryazev Institute of Plant PhysiologyRussian Academy of SciencesMoscowRussia

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