Russian Journal of Plant Physiology

, Volume 51, Issue 2, pp 164–168 | Cite as

Low-Temperature and Substrate Induction of the Gene for Δ9 Fatty Acid Desaturase in the Thermophilic Cyanobacterium Synechococcus vulcanus

  • D. A. Los


A decrease in ambient temperature induced the desC gene encoding the only acyl-lipid desaturase of thermophilic cyanobacterium Synechococcus vulcanus. The addition of the substrate for this enzyme, stearic acid (18:0), to the culture medium of cyanobacterium grown at optimum temperature also induced the desC gene. At 55 and 45°C, gene transcription started at the position –59 upstream of the translation initiation codon. After addition of 18:0, two specific pools of transcripts were detected. Some transcripts corresponded in size to transcripts synthesized at 55 and 45°C, and other transcripts were longer. The determination of sites of the transcript synthesis initiation showed that 18:0 additionally induced transcription from positions –163 and –169. These additional initiation sites were positioned within the coding sequence of the hemN gene preceding the desC gene. Thus, at low-temperature and substrate induction, the expression of the desC gene was evidently controlled by different promoter sequences.

Synechococcus vulcanus desaturases low-temperature regulation substrate regulation gene expression 


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© MAIK “Nauka/Interperiodica” 2004

Authors and Affiliations

  • D. A. Los
    • 1
  1. 1.Timiryazev Institute of Plant Physiology, Russian Academy of SciencesMoscowRussia

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