Abstract
One hundred strains of halophilic vibrios were isolated from 16 species of marine invertebrates of Peter the Great Bay. Based on their morphological and biochemical characteristics, the bacteria were identified as Vibrio parahaemolyticus and Vibrio alginolyticus. Bacterial isolates possessed virulence enzymes (DNAase, lecithinase, catalase) and were characterized by a high enterotoxigenicity. It was determined that 76% of the V. parahaemolyticus strains and 43% of the V. alginolyticus strains were Kanagawa-positive. The isolates showed a high adhesive capability, the average adhesion index was 18.06 cells per erythrocyte for V. parahaemolyticus and 12.55 for V. alginolyticus. The results of this study suggest a high pathogenic potential of the isolated halophilic vibrios, which are an epidemic hazard to marine invertebrates and to humans.
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REFERENCES
Alekseenko, V.V., Dobroshtan, E.V., Moiseeva, A.V., et al., Kishechnye infektsii (Intestinal Infections), Kiev: Naukova Dumka, 1991, no. 22, pp. 91–95.
Brilis, V.I., Brilene, T.A., Lentsner, Kh, P., and Lentsner, A.A., A Method for the Study of Adhesion Process in Microorganisms, Lab. Delo, 1986, no. 4, pp. 210–212.
Grigoryev, Yu.I., Rasprostranenie galofil'nykh vibrionov v morskoi vode (Occurrence of Halophilic Vibrios in Sea Water), Moscow: Nauka, 1974.
Dalin, M.V. and Fish, N.G., Adgeziny mikroorganizmov (Adhesive Agents of Microorganisms), Moscow: Mir, 1985, p. 43.
Dolgova, T.G. and Khomenko, T.V., Assessment of the Pathegenicity and Persistence Factors of Halophilic Bacteria from an Epidemiological Standpoint, Tikhookean. Med. Zh., Spec. Issue, 2001, no. 2, pp. 119–120.
Metodicheskie ukazaniya po laboratornoi diagnostike, epidemiologii, klinike, lecheniyu i profilaktike zabolevanii vyzyvaemykh paragemoliticheskimi i drugimi uslovno-patogennymi morskimi vibrionami (Methodological Guidelines on Laboratory Diagnostics, Epidemiology, Clinic, Treatment and Prophylaxis of Diseases Caused by Parahemolytic and other Conditionally Pathogenic Marine Vibrios), Department of Quarantine Infections of USSR Ministry of Health, 1985.
Pasternak, N.A., Andrusenko, I.T., Vedmina, E.A., Gabrielyan, S.A., et al., A Method for Determination of Enterotoxigenicity of Some Species of Gram-negative Bacteria, Lab. Delo, 1985, no. 9, pp. 556–557.
Alsina, M. and Blanch, A.R., A Set of Keys for Biological Identification of Environmental Vibrio Species, J. Appl. Bacteriol., 1994, vol. 76, pp. 79–85.
Alsina, M. and Blanch, A.R., Improvement and Update of a Set of Keys for Biochemical Identification of Vibrio Species, J. Appl. Bacteriol., 1994, vol. 77, pp. 719–721.
Austin, B. and Austin, D.A., Bacterial Fish Pathogens, Diseases of Farmed and Wild Fish, Chichester, 1999.
Gomes-Gil, B., Tron-Mayen, L., Roque, A., et al., Species of Vibrio Isolated from Hepatopancreas, Haemolymph and Digestive Tract of a Population of Healthy Juvenile Penaeus vannamei, Aquaculture, 1998, vol. 163, pp. 1–9.
Hara-Kudo, J., Nishina, T., Na Ragawa, H., et al., Improved Method for Detection of Vibrio parahaemolyticus in Sea Food, Appl. Environ. Microbiol., 2001, vol. 67, pp. 5819–5823.
Joseph, S.W., Kaper, J.B., and Colwell, R.R., Vibrio parahaemolyticus and Related Halophilic Vibrios, CRC Crit. Rev. Microbiol., 1982, vol. 10, pp. 77–124.
Macian, M.C., Arias, C.R., Aznar, R., et al., Identification of Vibrio spp. (Other Than V. vulnificus) Recovered on CPC Agar from Marine Natural Samples, Int. Microbiol., 2000, vol. 3, pp. 51–53.
Olafsen, J.A., Interactions between Fish Larvae and Bacteria in Marine Aquaculture, Aquaculture, 2001, vol. 200, pp. 223–247.
Olafsen, J.A., Mikkelsen, H.V., Giaever, H.M., and Hansen G.H., Indigenous Bacteria in Hemolymph and Tissues of Marine Bivalves at Low Temperature, Appl. Environ. Microbiol., 1993, vol. 59, pp. 1848–1854.
Ortigosa, M., Esteve, C., and Pujalte, M.J., Vibrio species in Seawater and Mussels: Abundance and Numerical Taxonomy, Syst. Appl. Microbiol., 1989, vol. 12, pp. 316–325.
Sakazaki, R., Iwanami, S., and Fukumi, H., Studies on Enteropathogenic, Facultatively Halophilic Bacteria Vibrio parahaemolyticus. I. Morphological, Cultural and Biochemical Properties and Its Taxonomical Position, Jap. J. Med. Sci. Biol., 1963, vol. 16, p. 161.
Smibert, R.M. and Krieg, N.R., Methods of General and Molecular Bacteriology, Washington: American Society for Microbiology, 1994, pp. 607–654.
The Prokaryotes. A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, Berlin: Springer-Verlag, 1992, vol. 2–3, pp. 2938–3011.
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Beleneva, I.A., Maslennikova, E.F. & Magarlamov, T.Y. Physiological and Biochemical Characteristics of the Halophilic Bacteria Vibrio parahaemolyticus and V. alginolyticus Isolated from Marine Invertebrates of Peter the Great Bay, Sea of Japan. Russian Journal of Marine Biology 30, 96–100 (2004). https://doi.org/10.1023/B:RUMB.0000025985.38429.11
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DOI: https://doi.org/10.1023/B:RUMB.0000025985.38429.11