Abstract
Analysis of the phenotypic manifestation of inactivation of several genes from the ytlI–ytnM operon containing the ribR gene and results of Northern hybridization showed that theribR gene does not have the self promoter and is transcribed from the main promoter of the ytlI–ytnM operon. Two sites of single nucleotide substitutions leading to derepression of ribR gene were identified between the putative main promoter and transcription start of the ytlI–ytnM operon regulatory region.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Mironov, A.S., Gusarov, I., Rafikov, R., et al., Sensing Small Molecules by Nascent RNA: A Mechanism to Control Transcription in Bacteria, Cell (Cambridge, Mass.), 2002, vol. 111, pp. 747-756.
Kreneva, R.A., Solovieva, I.M., Errais, L.L., et al.,. Investigation of the Regulatory Mechanism of the ribC Gene Activity in Bacillus subtilis, Genetika (Moscow), 2001, vol. 37,no. 9, pp. 1300-1303.
Kunst, F., Ogasavara, N., Moszer, I., et al., The Complete Genome Sequence of the Gram-Positive Bacterium Bacillus subtilis, Nature, 1997, vol. 390, pp. 249-256.
Kreneva, R.A. and Perumov, D.A., Genetic Mapping of an Additional Regulatory Locus of the Bacillus subtilis Riboflavin Operon, Genetika (Moscow), 1996, vol. 32,no. 12, pp. 1623-1628.
Solovieva, I.M., Kreneva, R.A., Leak, D.J., and Perumov, D.A., The ribR Gene Encodes a Monofunctional Riboflavin Kinase Which Is Involved in Regulation of the Bacillus subtilis Riboflavin Operon, Microbiology, 1999, vol. 145, pp. 67-73.
Coppee, J.Y., Auger, S., Turlin, E., et al., Sulfur-Limitation-Regulated Proteins in Bacillus subtilis: A Two-Dimensional Gel Electrophoresis Study, Microbiology, 2001, vol. 147, pp. 1631-1640.
Bresler, S.E., Glazunov, E.A., Chernik, T.P., et al., The Bacillus subtilis Riboflavin Biosynthesis Operon: Flavin Mononucleotide and Flavin Adenine Dinucleotide as Effectors of the Fiboflavin Operon, Genetika (Moscow), 1976, vol. 12,no. 5, pp. 83-91.
Vagner, V., Dervyn, E., and Ehrlich, S.D., A Vector for Systematic Gene Inactivation in Bacillus subtilis, Microbiology, 1998, vol. 144, pp. 3097-3104.
Kreneva, R.A. and Perumov, D.A., Genetic Mapping of Regulatory Mutations of Bacillus subtilis Riboflavin Operon, Mol. Gen. Genet., 1990, vol. 222, pp. 467-469.
Saito, H. and Miura, K.I., Preparation of Transforming DNA by Phenol Treatment, Biochim. Biophys. Acta, 1963, vol. 42, pp. 619-629.
Solovieva, I.M., Tarasov, K.V., and Perumov, D.A., Main Characteristics of Monofunctional Flavin Kinase of Bacillus subtilis, Biokhimiya, 2003, vol. 68,no. 2, pp. 212-217.
Sanger, F., Nicklen, S., and Coulson, A.R., DNA Sequencing with Chain-Terminating Inhibitors, Proc. Natl. Acad. Sci. USA, 1977, vol. 74, pp. 5463-5467.
Kil, Y.V., Mironov, V.N., Gorisin, I.Yu., et al., Riboflavin Operon in Bacillus subtilis: Unusual Symmetric Arrangement of the Regulatory Region, Mol. Gen. Genet., 1990, vol. 222, pp. 467-469.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Solovieva, I.M., Kreneva, R.A., Errais, L.L. et al. Study of the Mechanism for Regulating ribR Gene Activity in Bacillus subtilis . Russian Journal of Genetics 40, 580–583 (2004). https://doi.org/10.1023/B:RUGE.0000029163.22026.d0
Issue Date:
DOI: https://doi.org/10.1023/B:RUGE.0000029163.22026.d0