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Cytogenetic Maps of Lampbrush Chromosomes of Newts of the Genus Pleurodeles: An Algorithm of Lampbrush Chromosome Identification inPleurodeles waltl by Immunocytochemical Staining of Landmark Loops with Polyclonal Anti-Ro52 Antisera

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Abstract

Our work was aimed at developing a simple and effective method of identification of most or all chromosomes of Pleurodelesnewts. To this end, we used DAPI staining of the chromomeres of newt lampbrush chromosomes and immunochemical reactions between the ribonucleoproteins of landmark lateral loops and polyclonal antibodies against human zinc-finger protein Ro52 (52-kDa Ro/SS-A). A method has been developed to obtain lampbrush chromosome preparations in newts of the genes Pleurodeles. Cytological maps of P. waltl chromosomes (Spanish population/subspecies) showing distributions of chromomeres and marker landmark loops along the chromosome length were constructed.

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Authors and Affiliations

  1. Biological Institute, St. Petersburg State University, Staryi Peterhof, St. Petersburg, 198504, Russia

    N. M. Vishnyakova & A. V. Rodionov

  2. Universite Pierre et Marie Curie, Paris, France

    N. M. Vishnyakova & J.-C. Lacroix

Authors
  1. N. M. Vishnyakova
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  2. J.-C. Lacroix
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  3. A. V. Rodionov
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Vishnyakova, N.M., Lacroix, JC. & Rodionov, A.V. Cytogenetic Maps of Lampbrush Chromosomes of Newts of the Genus Pleurodeles: An Algorithm of Lampbrush Chromosome Identification inPleurodeles waltl by Immunocytochemical Staining of Landmark Loops with Polyclonal Anti-Ro52 Antisera. Russian Journal of Genetics 40, 491–499 (2004). https://doi.org/10.1023/B:RUGE.0000029150.41324.27

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