Abstract
Using computer-aided genomic methods, a complete map of the expressed sequence tags (EST) located in the human genome region 13q14.3 between the STS markers, D13S810 and D13S1469, was constructed. A total of 62 EST clusters were formed, of which 12 clusters corresponded to the already known human genes, 4 clusters represented pseudogenes, and 10 clusters were new human genes. The use of the method of reverse transcription in combination with polymerase chain reaction (RT–PCR) provided experimental confirmation of the existence of mRNA transcripts for the novel human genes revealed in silico.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Venter, J.C., Adams, M.D., Myers, E.W., et al., The Sequence of the Human Genome, Science, vol. 291, pp. 1304-1351.
Lander, E.S., Linton, L.M., Barren, B., et al., Initial Sequencing and Analysis of the Human Genome, Nature (London), vol. 409, pp. 860-921.
Baranova, A.V., Ivanov, D.V., Makeeva, N.V., et al., Cloning of a Candidate Leu5 Gene Lost in Patients with B-Cell Chronic lymphocytic leukemia as an Illustration of a Current Strategy of Search for Genes Suppressing Tumor Growth, Gematologiya i transfuziologiya, 2000, vol. 45,no. 3, pp. 15-19.
Cher, M.L., Bova, G.S., Moore, D.H., et al., Genetic Alterations in Untreated Metastases and Androgen-Independent Prostate Cancer Detected by Comparative Genomic Hybridization and Allelotyping, Cancer Res., 1996, vol. 56, pp. 3091-3102.
Zojer, N., Konigsberg, R., Ackermann, J., et al., Deletion of 13q14 Remains an Independent Adverse Prognostic Variable in Multiple Myeloma Despite Its Frequent Detection by Interphase Fluorescence in Situ Hybridization, Blood, 2000, vol. 95, pp. 1925-1930.
Tamura, K., Zhang, X., Murakami, Y., et al., Deletion of Three Distinct Regions on Chromosome 13q in Human Non-Small-Cell Lung Cancer, Int. J. Cancer, 1997, vol. 74, pp. 45-49.
Fogt, F., Zhuang, Z., Linehan, W.M., et al., Collecting Duct Carcinomas of the Kidney: A Comparative Loss of Heterozygosity Study with Clear Cell Renal Cell Carcinoma, Oncol. Rep., 1998, vol. 5, pp. 923-926.
Boguski, M.S., Lowe, T.M., and Tolstoshev, C.M., DbEST-Database for “Expressed Sequence Tags,” Nat. Genet., 1993, vol. 4, pp. 332-333.
Altschul, S.F., Gish, W., Miller, W., et al., Basic Local Alignment Search Tool, J. Mol. Biol., 1990, vol. 215, pp. 403-410.
Tyazhelova, T.V., Ivanov, D.V., Makeeva, N.V., et al., The Transcription Map of the 13q14 Region Frequently Deleted in B-Ccell Chronic Lymphocytic Leukemia, Russ. J. Genet., 2001, vol. 37,no. 11, pp. 1286-1292.
Hattori, M., Fujiyama, A., Taylor, T.D., et al., The DNA Sequence of Human Chromosome 21, Nature, 2000,. vol. 405, pp. 311-319.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tyazhelova, T.V., Ivanov, D.V., Nazarenko, S.A. et al. In silico Search for the Expressed Sequences in the Region q14.3 of Human Chromosome 13. Russian Journal of Genetics 40, 332–335 (2004). https://doi.org/10.1023/B:RUGE.0000021636.40144.5c
Issue Date:
DOI: https://doi.org/10.1023/B:RUGE.0000021636.40144.5c