Abstract
Donor strains of the Hfr type were isolated using plasmid pRK2013 with transposons Tn10 and B21 as a chromosome-mobilizing factor. The isolated strains were shown to promote transfer of donor chromosome from different origins in different directions during isogenic matings of Pseudomonas mendocina bacteria. The created collection of donors and polyauxotrophic recipient bacteria permitted mapping 26 genetic determinants on the bacterial chromosome and identifying the genome of these microorganisms as a circular DNA molecule.
Similar content being viewed by others
References
Reimmann, C. and Haas, D., IS21 Insertion in the trfA Replication Control Gene of Chromosomally Integrated Plasmid RP1: A Property of Stable Pseudomonas aeruginosa Hfr-Strains, Mol. Gen. Genet., 1986, vol. 23, pp. 511–519.
Morgan, A.F. and Dean, H.F., Chromosomal Map of Pseudomonas putida PPN, and a Comparison of Gene Order with the Pseudomonas aeruginosa PAO Chromosomal Map, J. Gen. Microbiol., 1985, vol. 67, pp. 885–896.
Herrmann, H., Klopotowski, T., and Gunter, E., The Hfr-Status of Pseudomonas aeruginosa Is Stabilized by Integrative Suppression, Mol. Gen. Genet., 1986, vol. 24, pp. 519–523.
Vasylenko, S.L., Titok, M.A., and Maximova, N.P., Construction of System of Genetic Analysis in Pseudomonas mendocina, Genetika (Moscow), 2000, vol. 36, no. 1, pp. 28–34.
Miller, J., Experiments in Molecular Genetics, Cold Spring Harbor, New York: Cold Springs Harbor Lab., 1976.
Vasylenko, S.L., Maximova, N.P., and Titok, M.A., Optimization of Mutagenesis Conditions for Bacteria Pseudomonas mendocina, Vestn. Belorus. Gos. Univ., Ser. 2, 2003, no. 1, pp. 43–47.
Davis, R., Botstein, D., and Roth, J., A Manual for Genetic Engineering: Advanced Bacterial Genetics, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1982, pp. 146–148.
Yanofsky, C., Enzymatic Studies with a Series of Tryptophan Auxotrophs of Escherichia coli, J. Biol. Chem., 1957, vol. 224, p. 783.
Figurski, D.H. and Helinski, D.R., Replication of an Origin-Containing Derivative of Plasmid RK2 Dependent on a Plasmid Function Provided in Trans, Proc. Natl. Acad. Sci. USA, 1979, vol. 76, pp. 1648–1652.
Simon, R., Quandt, J., and Klipp, W., New Derivatives of Transposon Tn5 Suitable for Mobilization of Replicons, Generation of Operon Fusions and Inductions of Genes in Gram-Negative Bacteria, Gene, 1989, vol. 80, pp. 161–169.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vasylenko, S.L., Maximova, N.P. & Titok, M.A. Conjugational Mapping of Bacteria Pseudomonas mendocina . Russian Journal of Genetics 39, 1219–1226 (2003). https://doi.org/10.1023/B:RUGE.0000004135.76637.8e
Issue Date:
DOI: https://doi.org/10.1023/B:RUGE.0000004135.76637.8e