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Extraction and identification of antioxidant components from Artemisia capillaris herba

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Abstract

The antioxidant activities of various organic solvent extracts from Artemisia capillaris herba were investigated. The results indicated that methanol extracts of Artemisia capillaris herba possessed higher contents of phenolic compounds and showed stronger antioxidant activities than other organic solvent extracts. The methanol extracts of Artemisia capillaris herba exhibited 91.1% of the scavenging effect at a dose of 200 ppm on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and showed strong antioxidant activity in the Β-carotene bleaching system. The methanol extracts were separated into three fractions by preparative HPLC using a Wakopak C18 reversed-phase column. After purifing with preparative HPLC, AC-1, 2 and 3 compounds were obtained. Their structures were established as chlorogenic acid (AC-1), 3,5-dicaffeoylquinic acid (AC-2) and 3,4-dicaffeoylquinic acid (AC-3) on the basis of UV-vis spectral, HPLC and FAB-MS data. The scavenging effect of purified compounds on DPPH radical equaled that of tert-butyl-4-hydroxytoluene (BHT) and α-tocopherol at a dose of 10 ppm.

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Authors and Affiliations

  1. Graduate School of Biotechnology, Korea University, 1, 5-ka, Anam-dong, Sungbuk-ku, Seoul, 136-701, Korea

    Ho-Chan Seo & Jae-Ho Kim

  2. National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan

    Masahiro Suzuki & Mayumi Ohnishi-Kameyama

  3. Department of Aqualife Medicine, Yosu National University, 195 Guk-dong, Yosu city, 550-749, Chollanam-do, Korea

    Myung-Joo Oh

  4. Faculty of Science and Biotechnology, Pukyong National University, Daeyeon 3-dong, Nam-ku, Pusan, 608-737, Korea

    Hyeung-Rak Kim

  5. National Institute of Animal Industry, Ikenodai, Kukizakicho, Inashiki gun, Ibaraki, 305-0901, Japan

    Tadahiro Nagata

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  1. Ho-Chan Seo
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  2. Masahiro Suzuki
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  5. Hyeung-Rak Kim
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  6. Jae-Ho Kim
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  7. Tadahiro Nagata
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Seo, HC., Suzuki, M., Ohnishi-Kameyama, M. et al. Extraction and identification of antioxidant components from Artemisia capillaris herba . Plant Foods Hum Nutr 58, 1–12 (2003). https://doi.org/10.1023/B:QUAL.0000040355.60922.08

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  • DOI: https://doi.org/10.1023/B:QUAL.0000040355.60922.08

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