Abstract
Defining a quantitative relationship between chlorophyll a fluorescence yield and Photosystem II (PS II) function is important to photosynthesis research. Prior work [Peterson and Havir (2003) Photosynth Res 75: 57–70] indicated an apparent effect of psbS genotype on the in vivo rate constant for photochemistry in PS II (kP0). The nuclear psbS gene encodes a 22-kDa pigment-binding antenna protein (PS II-S) essential for photoprotective nonphotochemical quenching (NPQ) in PS II. Ten Arabidopsis thaliana lines were chosen for study, encompassing effects on PS II-S expression level and/or structure due to single-site amino acid substitution. Short-term (i.e. seconds) irradiance-dependent changes in steady state fluorescence yields Fo and Fm(open and closed centers, respectively) were evaluated for compliance with the reversible radical pair (RRP) model of PS II. All lines (including normal Nicotiana tabacum and Zea mays) deviated from the RRP scheme in the same way indicating that psbS genotype per se does not alter interactions between the antenna and reaction center and thereby affect kP0. Rather, observed departures from RRP model behavior are consistent with overestimation of Fm due to perturbing effects of the saturating multiple turnover flash employed in its measurement. Reversal of direct quenching of singlet states by plastoquinone during the flash could occur but by itself cannot account for the anomalous covariation in Fo and Fm. Reduction of the PS II acceptor side apparently either amplifies the rate constant for fluorescence or suppresses that of xanthophyll-dependent thermal deactivation (qE). A procedure was devised that considers Fo when correcting maximal fluorescence values for measurement bias. A high degree of consistency in assessment of PS II quantum yield based on corrected fluorescence parameters and simultaneous CO2 exchange measurements was noted under both steady state and transient conditions (360 μl CO2l−1, 1% O2).
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References
Aspinall-O'Dea M, Wentworth M, Robert B, Ruban A and Horton P (2002) In vitro reconstitution of the activated zeaxanthin state associated with energy dissipation in plants. Proc Natl Acad Sci USA 99: 16331–16335
Dau H (1994) Molecular mechanisms and quantitative models of variable Photosystem II fluorescence. Photochem Photo-biol 60: 1–23
Delosme R (1967) Étude de l 'induction de fluorescence des algues vertes et des chloroplastes au de ´but d 'une illumination intense. Biochim Biophys Acta 143: 108–128
Dreuw A, Fleming GR and Head-Gordon M (2003) Chloro-phyll fluorescence quenching by xanthophylls. Phys Chem Chem Phys 5: 3247–3256
Funk C, Schrőder WP, Napiwotzki A, Tjus SE, Renger G and Andersson B (1995) The PS II-S protein of higher plants: a new type of pigment-binding protein. Biochemistry 34: 11133–11141
Genty B, Briantais J-M and Baker NR (1989) The relationship between the quantum yield of photosynthetic electron trans-port and quenching of chlorophyll fluorescence. Biochim Biophys Acta 990: 87–92
Genty B, Wonders J and Baker NR (1990) Non-photochemical quenching of Fo in leaves is emission wavelength dependent: consequences for quenching analysis and its interpretation. Photosynth Res 26: 133–139
Heber U (2002) Irrungen, Wirrungen?The Mehler reaction in relation to cyclic electron transport in C3 plants. Photosynth Res 73: 223–231
Krause GH and Weis E (1991) Chlorophyll fluorescence and photosynthesis: the basics. Annu Rev Plant Physiol Plant Mol Biol 42: 313–349
Laisk A and Loreto F (1996) Determining photosynthetic parameters from leaf CO2 exchange and chlorophyll fluores-cence: Rubisco speci city factor, dark respiration in the light, excitation distribution between photosystems, alternative electron transport and mesophyll diffuusion resistance. Plant Physiol 110: 903–912
Laisk A and Oja V (1998) Dynamics of Leaf Photosynthesis. Rapid Response Measurements and Their Interpretations. CSIRO, Collingwood, Australia
Laisk A, Oja V, Rasulov B, Eichelmann H and Sumberg A (1997) Quantum yields and rate constants of photochemical and nonphotochemical excitation quenching. Experiment and model. Plant Physiol 115: 803–815
Li X-P, Bjőrkman O, Shih C, Grossman AR, Rosenquist M, Jansson S and Niyogi KK (2000) A pigment-binding protein essential for regulation of photosynthetic light harvesting. Nature 403: 391–395
Li X-P, Phippard A, Pasari J and Niyogi KK (2002) Structure-function analysis of Photosystem II subunit S (PsbS)in vivo. Funct Plant Biol 29: 1131–1139
Ma Y-Z, Holt NE, Li X-P, Niyogi KK and Fleming GR (2003) Evidence for direct carotenoid involvement in the regulation of photosynthetic light harvesting. Proc Natl Acad Sci USA 100: 4377–4382
Niyogi KK (1999) Photoprotection revisited: genetic and molecular approaches. Annu Rev Plant Physiol Plant Mol Biol 50: 333–359
Noctor G and Foyer CH (1998) A re-evaluation of the ATP: NADPH budget during C3 photosynthesis: a contri-bution from nitrate assimilation and its associated respiratory activity? J Exp Bot 49: 1895–1908
Oja V, Eichelmann H, Peterson RB, Rasulov B and Laisk A (2003) Deciphering the 820 nm signal: redox state of donor side and quantum yield of Photosystem I in leaves. Photo-synth Res 78: 1–15
Peterson RB and Havir EA (2001) Photosynthetic properties of an Arabidopsis thaliana mutant possessing a defective psbS gene. Planta 214: 142–152
Peterson RB and Havir EA (2003) Contrasting modes of regulation of PS II light utilization with changing irradiance in normal and psbS mutant leaves of Arabidopsis thaliana. Photosynth Res 75: 57–70
Peterson RB, Oja V and Laisk A (2001) Chlorophyll fluores-cence at 680 and 730 nm and leaf photosynthesis. Photosynth Res 70: 185–196
Ruuska SA, Badger MA, Andrews JT and von Caemmerer S (2000) Photosynthetic electron sinks in transgenic tobacco with reduced amounts of Rubisco: little evidence for significant Mehler reaction. J Exp Bot: 51: 357–368
Samson G and Bruce D (1996) Origins of the low yield of chlorophyll a fluorescence induced by single turnover flashes in spinach thylakoids. Biochim Biophys Acta 1276: 147–153
Samson G, Prášil O and Yaakoubd B (1999) Photochemical and thermal phases of chlorophyll a fluorescence. Photosyn-thetica 37: 163–182
Schatz GH, Brock H and Holzwarth AR (1988) Kinetic and energetic model for the primary processes of Photosystem II. Biophys J 54: 397–405
Schreiber U, Hormann H, Neubauer C and Klughammer C (1995) Assessment of Photosystem II photochemical quan-tum yield by chlorophyll fluorescence quenching analysis. Aust J Plant Physiol 22: 209–220
Schreiber U and Neubauer C (1990) O2-dependent electron flow, membrane energisation and the mechanism of nonphoto-chemical quenching of chlorophyll fluorescence. Photosynth Res 25: 279–293
Seaton GGR and Walker DA (1990) Chlorophyll fluorescence as a measure of photosynthetic carbon assimilation. Proc R Soc London 242: 29–35
Stewart DH and Brudvig GW (2001) Cytochrome b559 of Photosystem II. Biochim Biophys Acta 1367: 63–87
Strasser RJ, Srivastava A and Govindjee (1995) Polyphasic chlorophyll a fluorescence transient in plants and cyanobac-teria. Photochem Photobiol 61: 32–42
Vasil'ev S and Bruce D (1998) Nonphotochemical quenching of excitation energy in Photosystem II. A picosecond time-resolved study of the low yield of chlorophyll a fluorescence induced by single-turnover flash in isolated spinach thylak-oids. Biochemistry 37: 11046–11054
Vernotte C, Etienne AL and Briantais J-M (1979) Quenching of the system II chlorophyll fluorescence by the plastoquinone pool. Biochim Biophys Acta 545: 519–527
Weis E and Lechtenberg D (1989) Fluorescence analysis during steady-state photosynthesis. Phil Trans R Soc London Ser B 323: 252–268
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Peterson, R.B., Havir, E.A. The Multiphasic Nature of Nonphotochemical Quenching: Implications for Assessment of Photosynthetic Electron Transport Based on Chlorophyll Fluorescence. Photosynthesis Research 82, 95–107 (2004). https://doi.org/10.1023/B:PRES.0000040477.43858.54
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DOI: https://doi.org/10.1023/B:PRES.0000040477.43858.54