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X-Ray Structure of Rhodobacter Capsulatus Cytochrome bc 1: Comparison with its Mitochondrial and Chloroplast Counterparts

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Abstract

Ubihydroquinone: cytochrome (cyt)c oxidoreductase, or cyt bc 1, is a widespread, membrane integral enzyme that plays a crucial role during photosynthesis and respiration. It is one of the major contributors of the electrochemical proton gradient, which is subsequently used for ATP synthesis. The simplest form of the cyt bc 1 is found in bacteria, and it contains only the three ubiquitously conserved catalytic subunits: the Fe–S protein, cyt b and cyt c 1. Here we present a preliminary X-ray structure of Rhodobacter capsulatus cyt bc 1 at 3.8 Å and compare it to the available structures of its homologues from mitochondria and chloroplast. Using the bacterial enzyme structure, we highlight the structural similarities and differences that are found among the three catalytic subunits between the members of this family of enzymes. In addition, we discuss the locations of currently known critical mutations, and their implications in terms of the cyt bc 1 catalysis.

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  1. Maria Valkova-Valchanova

    Present address: , Medarex, Inc, Bloomsbury, NJ, 08804, USA

Authors and Affiliations

  1. Laurence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA, 94720, USA

    Edward A. Berry, Li-Shar Huang, Lai K. Saechao & Ning. G. Pon

  2. University of Pennsylvania, Department of Biology, Philadelphia, PA, 19104-6018, USA

    Maria Valkova-Valchanova & Fevzi Daldal

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  1. Edward A. Berry
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  2. Li-Shar Huang
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  3. Lai K. Saechao
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  4. Ning. G. Pon
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  5. Maria Valkova-Valchanova
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  6. Fevzi Daldal
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Correspondence to Edward A. Berry.

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Berry, E.A., Huang, LS., Saechao, L.K. et al. X-Ray Structure of Rhodobacter Capsulatus Cytochrome bc 1: Comparison with its Mitochondrial and Chloroplast Counterparts. Photosynthesis Research 81, 251–275 (2004). https://doi.org/10.1023/B:PRES.0000036888.18223.0e

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