Abstract
The present study was conducted to observe the role of the root—shoot transition zone in the development of PS I and PS II in red light. The development of PS II and PS I was severely inhibited when root—shoot transition zones of wheat seedlings were exposed to red light (670 nm) of intensity 500 µmol m−2 s−1. Chlorophyll biosynthesis was also inhibited in these seedlings. Most of the PS I and PS II proteins (D1, LHCPII, CP47, OEC33) and their transcript levels were severely inhibited but cyt b6f complex proteins were only partially inhibited. Protein and transcript levels of Rubisco large subunit and protochlorophyllide (Pchlide) biosynthesis were also severely inhibited in these seedlings. When incubated in the dark with or without the precursor of chlorophyll biosynthesis ALA, these plants accumulated most of the Pchlide, as non-phototransformable Pchlide, suggesting low activity of NADPH:protochlorophyllide oxidoreductase (EC 1.6.99.1) in these plants. These effects were not observed when the seedlings were grown in red light with their root—shoot transition zones covered. These results suggest that the root—shoot transition zone plays an important role in the overall greening process involving transcription and translation of photosynthetic genes.
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References
Bovery F, Ogawa T and Shibata K (1974) Photoconvertible and non-photoconvertible forms of protochlorophyll(ide) in etiolated bean leaves. Plant Cell Physiol 15: 1133–1137
Buschmann C, Meiger D, Kleudgen HK and Lichtenthaler HK (1978) Regulation of chloroplast development by red and blue light. Photochem Photobiol 27: 195–198
Chomczynski P and Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159
Chrispeels HE, Oettinger H, Janvier N and Tague BW (2000) AtZFP1, encoding Arabidopsis thaliana C2H2 zinc-finger protein 1, is expressed downstream of photomorphogenic activation. Plant Mol Biol 42: 279
Eskins K and Duysen M (1984) Chloroplast structure in normal and pigment-deficient soyabean grown in continuous red and far-red light. Physiol Plant 61: 351–356
Eskins K, McCarthy SA, Dybas L and Duysen M (1986) Corn chloroplast development in weak fluence rate red light and in weak fluence rate red plus far-red light. Physiol Plant 67: 242–246
Fankhauser C and Chory J (1997) Light control of plant development. Ann Rev Cell Dev Biol 13: 203–229
Franck F, Bereza B and Böddi B (1999) Protochlorophyllide—NADP+ and protochlorophyllide—NADPH complexes and their regeneration after flash illumination in leaves and etioplast membranes of dark-grown wheat. Photosynth Res 59: 53–61
Herrin DL, Battey JF, Greer K and Schmidt GW (1992) Regulation of chlorophyll apoprotein expression and accumulation. J Biol Chem 267: 8260–8269
Ivanov AG, Morgan RM, Gray GR, Vetlikova MY and Huner NPA (1998) Temperature/light dependent development of selective resistance to photoinhibition of Photosystem I. FEBS Lett 430: 288–292
Jilani A, Santosh K, Bose S and Tripathy BC (1996) Regulation of the carotenoid content and chloroplast development by levulinic acid. Physiol Plant 96: 139–145
Johanningmeier U and Howell SH (1984) Regulation of light-harvesting chlorophyll binding protein mRNA accumulation in Chlamydomonas reinhardtii. J Biol Chem 259: 13541–13549
Kapoor S, Maheshwari SC and Tyagi AK (1994) Developmental and light-dependent cues interact to establish steady-state levels of transcripts for photosynthesis-related genes (psbA, psbD, psaA and rbcL) in rice (Oryza sativa L.). Curr Genet 25: 362–366
Klein RR and Mullet JE (1986) Regulation of chloroplast-encoded-chlorophyll-binding protein translation during higher plant chloroplast biogenesis. J Biol Chem 261: 11138–11145
Klein RR, Mullet JE (1987) Control of gene expression during higher plant chloroplast biogenesis. J Biol Chem 262: 4341–4348
Laemalli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
McCree KJ (1972) Test of current definitions of photosynthetically active radiation against leaf photosynthesis data. Agr Meteorol 10: 443–453
McEwen B, Virgin HI, Boddi B and Sundqvist C (1991) Protochlorophyll forms in roots of dark-grown plants. Physiol Plant 81: 455–461
Mullet JE and Klein RR (1987) Transcription and RNA stability are important determinants of higher plant chloroplast RNA levels. EMBO J 6: 1571–1579
Mullet JE, Klein PG and Klein RR (1990) Chlorophyll regulates accumulation of the plastid-encoded chlorophyll apoproteins CP43 and D1 by increasing apoprotein stability. Proc Natl Acad Sci USA 87: 4038–4042
Mustardy L, Cunningham Jr FX and Gannt E (1992) Photosynthetic membrane topography: quantitative in situ localization of Photosystems I and II. Proc Natl Acad Sci USA 89: 10021–10025
Ouazzani CAM, Schoefs B and Franck F (1998) Isolation and characterization of photoactive complexes of NADPH protochlorophyllide oxidoreductase. Planta 206: 673–680
Pfannschmidt T, Schutze K, Brost M and Oelmuller R (2001) A novel mechanism of nuclear photosynthesis gene regulation by redox signals from the chloroplast during photosystem stoichiometry adjustment. J Biol Chem 276: 36125–36130
Schafer E and Mohr H (1974) Irradiance dependency of the phytochrome systems in cotyledons of mustard (Synapis alba L.). J Math Biol 1: 9–15
Schoefs B and Bertrand M (2000) The formation of chlorophyll from chlorophyllide in leaves containing proplastids is a four-step process. FEBS Lett 486: 243–246
Schoefs B, Bertrand M and Franck F (2000a) Spectroscopic properties of protochlorophyllide analyzed in situ in the course of etiolation and in illuminated leaves. Photochem Photobiol 72: 85–93
Schoefs B, Bertrand M and Franck F (2000b) Photoactive protochlorophyllide regeneration in cotyledons and leaves from higher plants. Photochem Photobiol 72: 660–668
Shibata K (1957) Spectroscopic studies of chlorophyll formation in intact leaves. J Biochem (Tokyo) 44: 147–173
Sirnoval C, Kuyer Y, Michel JM and Brouers M (1967) The primary photoact in the conversion of protochlorophyllide into chlorophyllide. Stud Biophys 5: 43–50
Sood S and Tripathy BC (1998) Etiolation response of wheat seedlings grown under red light. In: Photosynthesis: Mechanisms and Effects, Proceedings of XIth International Congress on Photosynthesis, Budapest, Hungary, 17–22 August 1998. Kluwer Academic Publishers, Dordrecht, The Netherlands
Sutton A, Sieburth LE and Benett J (1987) Light-dependent accumulation and localization of Photosystem II proteins in maize. Eur J Biochem 164: 571–578
Tanaka A, Yamamoto Y and Tsuji H (1991) Formation of chlorophyll-protein complexes during greening. 2. Redistribution of chlorophyll among apoproteins. Plant Cell Physiol 32: 195–204
Thorne SW (1971) The greening of etiolated bean leaves I. The initial photoconversion process. Biochim Biophys Acta 226: 113–127
Towbin H, Staehelin T and Gordn J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354
Tripathy BC and Brown CS (1995) Root-shoot interaction in the greening of wheat seedlings grown under red light. Plant Physiol 107: 407–411
Tripathy BC and Mohanty P (1980) Zinc-inhibited electron transport of photosynthesis in isolated barley chloroplasts. Plant Physiol 66: 1174–1178
Vasilikiotis C and Melvis A (1994) Photosystem II reaction center damage and repair cycle: chloroplast acclimation strategy to irradiance stress. Proc Natl Acad Sci USA 91: 7222–7226
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Sood, S., Tyagi, A. & Tripathy, B. Inhibition of Photosystem I and Photosystem II in Wheat Seedlings with their Root–shoot Transition Zones Exposed to Red Light. Photosynthesis Research 81, 31–40 (2004). https://doi.org/10.1023/B:PRES.0000028337.72340.3a
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DOI: https://doi.org/10.1023/B:PRES.0000028337.72340.3a